Chemerin Kit ELISA

N° du produit ABIN2859336

Détail du produit Chemerin Kit ELISA

Antigène: Chemerin (RARRES2) (Retinoic Acid Receptor Responder (Tazarotene Induced) 2 (RARRES2))

Épitope: AA 20-162

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.78-50 ng/mL

Seuil minimal de détection: 0.78 ng/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Chemerin/RARRES2

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli
Immunogen sequence: T20-K162

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <20pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: E.coli
Immunogen sequence: T20-K162

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Tissue Specificity: Expressed in the differentiated adipocytes (at protein level). Abundantly expressed in the liver, adipose tissue including visceral, epididymal, and brown adipose tissue. .

Plaque: Pre-coated

Protocole: mouse Chemerin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Chemerin has been precoated onto 96-well plates. Standards(E.coli, T20-K162) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Chemerin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Chemerin amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL mouse Chemerin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse Chemerin standard solution and each sample is measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(ng/ml): 3.9, Standard deviation: 0.199, CV(%): 5.1
• Sample 2: n=16, Mean(ng/ml): 16, Standard deviation: 0.752, CV(%): 4.7
• Sample 3: n=16, Mean(ng/ml): 27, Standard deviation: 0.999, CV(%): 3.7,
• Sample 1: n=24, Mean(ng/ml): 4, Standard deviation: 0.34, CV(%): 8.5
• Sample 2: n=24, Mean(ng/ml): 17.8, Standard deviation: 1.23, CV(%): 6.9
• Sample 3: n=24, Mean(ng/ml): 30, Standard deviation: 1.62, CV(%): 5.4

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: Chemerin (RARRES2) (Retinoic Acid Receptor Responder (Tazarotene Induced) 2 (RARRES2))

Autre désignation: RARRES2 (RARRES2 Produits)

Synonymes: HP10433 Kit ELISA, TIG2 Kit ELISA, 0610007L05Rik Kit ELISA, AI303516 Kit ELISA, retinoic acid receptor responder 2 Kit ELISA, retinoic acid receptor responder (tazarotene induced) 2 Kit ELISA, RARRES2 Kit ELISA, Rarres2 Kit ELISA

Sujet:

Protein Function: Adipocyte-secreted protein (adipokine) that regulates adipogenesis, metabolism and inflammation through activation of the chemokine-like receptor 1 (CMKLR1). Its other ligands include G protein-coupled receptor 1 (GPR1) and chemokine receptor-like 2 (CCRL2). Positively regulates adipocyte differentiation, modulates the expression of adipocyte genes involved in lipid and glucose metabolism and might play a role in angiogenesis, a process essential for the expansion of white adipose tissue. Also acts as a proinflammatory adipokine, causing an increase in secretion of proinflammatory and prodiabetic adipokines, which further impair adipose tissue metabolic function and have negative systemic effects including impaired insulin sensitivity, altered glucose and lipid metabolism, and a decrease in vascular function in other tissues. Can have both pro- and anti-inflammatory properties depending on the modality of enzymatic cleavage by different classes of proteases. Acts as a chemotactic factor for leukocyte populations expressing CMKLR1, particularly immature plasmacytoid dendritic cells, but also immature myeloid DCs, macrophages and natural killer cells. Exerts an anti-inflammatory role by preventing TNF/TNFA-induced VCAM1 expression and monocytes adhesion in vascular endothelial cells. The effect is mediated via inhibiting activation of NF-kappa-B and CRK/p38 through stimulation of AKT1/NOS3 signaling and nitric oxide production. Exhibits an antimicrobial function in the skin. .

Background: Chemerin, also known as RARRES2 or TIG2, is a protein that is encoded by the RARRES2 gene. It is mapped to 7q36.1. Chemerin is a potent chemoattractant specific for antigen-presenting cells that requires proteolytic activation and acts as a ligand for the G protein-coupled receptor CMKLR1(also known as ChemR23). It is a 14 kDa protein secreted in an inactive form as prochemerin and is activated through cleavage of the C-terminus by inflammatory and coagulation serine proteases. Chemerin was found to stimulate chemotaxis of dendritic cells and macrophages to the site of inflammation. What’s more, the active protein has several roles, including that as an adipokine, and is truncated on both termini from the proprotein.

Synonyms: Retinoic acid receptor responder protein 2,Chemerin,Rarres2,

Full Gene Name: Retinoic acid receptor responder protein 2

Cellular Localisation: Secreted.

ID gène: 71660

UniProt: Q9DD06

Pathways: Brown Fat Cell Differentiation