Osteoactivin Kit ELISA

N° du produit ABIN2859337

Détail du produit Osteoactivin Kit ELISA

Antigène: Osteoactivin (GPNMB)

Épitope: AA 23-502

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156-10.000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Osteoactivin/GPNMB

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: K23-N502

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: K23-N502

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Belongs to the PMEL/NMB family.

Tissue Specificity: In early development, mRNA is detected at high levels in the outer layer of the retina. Later in development expression gets restricted to the retinal pigment epithelium and iris. Overexpressed in mammary cancer cells possessing a strong bone metastatic phenotype. .

Plaque: Pre-coated

Protocole: mouse Osteoactivin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Osteoactivin has been precoated onto 96-well plates. Standards(NSO, K23-N502) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Osteoactivin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Osteoactivin amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL mouse Osteoactivin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse Osteoactivin standard solution and each sample is measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 1174, Standard deviation: 64.57, CV(%): 5.5
• Sample 2: n=16, Mean(pg/ml): 3248, Standard deviation: 207.9, CV(%): 6.4
• Sample 3: n=16, Mean(pg/ml): 5532, Standard deviation: 271.1, CV(%): 4.9,
• Sample 1: n=24, Mean(pg/ml): 1354, Standard deviation: 82.6, CV(%): 6.1
• Sample 2: n=24, Mean(pg/ml): 3426, Standard deviation: 246.7, CV(%): 7.2
• Sample 3: n=24, Mean(pg/ml): 5840, Standard deviation: 315.4, CV(%): 5.4

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: Osteoactivin (GPNMB)

Autre désignation: GPNMB (GPNMB Produits)

Synonymes: HGFIN Kit ELISA, NMB Kit ELISA, DC-HIL Kit ELISA, Dchil Kit ELISA, ipd Kit ELISA, glycoprotein nmb Kit ELISA, glycoprotein (transmembrane) nmb Kit ELISA, glycoprotein nmb L homeolog Kit ELISA, Gpnmb Kit ELISA, GPNMB Kit ELISA, gpnmb.L Kit ELISA

Sujet:

Protein Function: Could be a melanogenic enzyme. .

Background: Transmembrane glycoprotein NMB is a protein that is encoded by the GPNMB gene. In osteoblast progenitor cells, GPNMB works as a positive regulator of osteoblast differentiation during later stages of matrix maturation and mineralization that is mediated at least in part by BMP-2 in a SMAD1 dependent manner to promote osteoblast differentiation. GPNMB can enhance the repairing process in bone fracture, demonstrated by its high expression during chondrogenesis(soft callus) and osteogenesis(hard callus) compared to the intact femurs that is why Osteoactivin(OA) could be a novel therapeutic agent used to treat generalized osteoporosis or localized osteopenia during fracture repair by stimulating bone growth and regeneration. Similarly, GPNMB expression increases during osteoclast differentiation and it is functionally implicated in this process, possibly by promoting the fusion of osteoclast progenitor cells.

Synonyms: Transmembrane glycoprotein NMB,DC-HIL,Dendritic cell-associated transmembrane protein,Osteoactivin,Gpnmb,Dchil, Hgfin, Nmb,

Full Gene Name: Transmembrane glycoprotein NMB

Cellular Localisation: Cell membrane, Single-pass type I membrane protein . Endomembrane system, Single-pass type I membrane protein . Cytoplasmic vesicle membrane, Single-pass type I membrane protein . Melanosome . Vesicular, endosomal like structures.

ID gène: 93695

UniProt: Q99P91