Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

MADCAM1 Kit ELISA

MADCAM1 Reactivité: Souris AA 22-365 Colorimetric Sandwich ELISA 125-8000 pg/mL Cell Culture Supernatant, Serum
N° du produit ABIN2859338
  • Antigène Voir toutes MADCAM1 Kits ELISA
    MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))
    Épitope
    AA 22-365
    Reactivité
    • 7
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    125-8000 pg/mL
    Seuil minimal de détection
    125 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse MADCAM-1
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: NSO
    Immunogen sequence: Q22-T365
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <10pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: NSO
    Immunogen sequence: Q22-T365
    Featured
    Discover our best selling MADCAM1 Kit ELISA
    Top Product
    Discover our top product MADCAM1 Kit ELISA
  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Tissue Specificity: Highly expressed on high endothelial venules (HEV) of organized intestinal lymphoid tissues like the Peyer patches and mesenteric lymph nodes, and in the lamina propria of the intestine. Some expression found in the spleen, and low levels of expression in the peripheral lymph nodes and the lactating mammary gland. No expression was detected in the liver, kidneys, lungs or in normal brain. Expressed as well in brain endothelioma cells, and mucosal tissues which are in a chronic state of inflammation, such as inflammed pancreas. .

    Plaque
    Pre-coated
    Protocole
    mouse MADCAM-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for MADCAM-1 has been precoated onto 96-well plates. Standards(NSO, Q22-T365) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MADCAM-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse MADCAM-1 amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 8000pg/mL, 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL mouse MADCAM-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse MADCAM-1 standard solution and each sample be measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(pg/ml): 853, Standard deviation: 53.74, CV(%): 6.3
    • Sample 2: n=16, Mean(pg/ml): 2254, Standard deviation: 133, CV(%): 5.9
    • Sample 3: n=16, Mean(pg/ml): 3827, Standard deviation: 183.7, CV(%): 4.8,
    • Sample 1: n=24, Mean(pg/ml): 976, Standard deviation: 75.15, CV(%): 7.7
    • Sample 2: n=24, Mean(pg/ml): 2486, Standard deviation: 164.1, CV(%): 6.6
    • Sample 3: n=24, Mean(pg/ml): 4127, Standard deviation: 210.5, CV(%): 5.1
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Antigène Voir toutes MADCAM1 Kits ELISA
    MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))
    Autre désignation
    MADCAM1 (MADCAM1 Produits)
    Synonymes
    MACAM1 Kit ELISA, AV211525 Kit ELISA, mucosal vascular addressin cell adhesion molecule 1 Kit ELISA, MADCAM1 Kit ELISA, Madcam1 Kit ELISA
    Sujet

    Protein Function: Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both the integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Both isoform 1 and isoform 2 can adhere to integrin alpha-4/beta-7. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding. .

    Background: MADCAM1(Mucosal Vascular Addressin Cell Adhesion Molecule 1), also known as MACAM1, is a protein that is encoded by the MADCAM1 gene. By PCR-based analysis of somatic cell hybrids, Leung et al(1997) mapped the MACAM1 gene to chromosome 19. The protein encoded by this gene is an endothelil cell adhesion molecule that interacts preferentially with the leukocyte beta7 integrin LPAM-1(alpha4 / beta7), L-selectin, and VLA-4(alpha4/beta1) on myeloid cells to direct leukocytes into mucosal and inflamed tissues. It is a member of the immunoglobulin superfamily and is similar to ICAM-1 and VCAM-1.

    Synonyms: Mucosal addressin cell adhesion molecule 1,MAdCAM-1, mMAdCAM-1,Madcam1,

    Full Gene Name: Mucosal addressin cell adhesion molecule 1

    Cellular Localisation: Membrane, Single-pass type I membrane protein.
    ID gène
    17123
    UniProt
    Q61826
Vous êtes ici:
Support technique