GHRH Kit FLISA
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- GHRH (Growth Hormone Releasing Hormone (GHRH))
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Reactivité
- Humain
- Méthode de détection
- Fluorometric
- Type de méthode
- Competition ELISA
- Application
- ELISA
- Analytical Method
- Quantitative
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Plaque
- Pre-coated
- Protocole
- The immunoplate in this kit is pre-coated with a secondary antibody and the nonspecific binding sites are blocked. The secondary antibody can bind to the Fc fragment of the primary antibody (peptide antibody) whose Fab fragment will be competitively bound by both biotinylated peptide and peptide standard or targeted peptide in samples. The biotinylated peptide interacts with streptavidin-horseradish peroxidase (SA-HRP) which catalyzes the substrate. The fluorescence intensity is directly proportional to the amount of biotinylated peptide-SA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples. This is due to the competitive binding of the biotinylated peptide with the standard peptide or samples to the peptide antibody (primary antibody). A standard curve of known concentration can be established accordingly. The unknown concentration in samples can be determined by extrapolation to this standard curve.
- Restrictions
- For Research Use only
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- Stock
- 4 °C
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- Antigène Voir toutes GHRH Kits FLISA
- GHRH (Growth Hormone Releasing Hormone (GHRH))
- Autre désignation
- Growth Hormone Releasing Factor (GHRF) (GHRH Produits)
- Synonymes
- GHRF Kit FLISA, GRF Kit FLISA, INN Kit FLISA, PACAP Kit FLISA, grf Kit FLISA, Ghrf Kit FLISA, growth hormone releasing hormone Kit FLISA, adenylate cyclase activating polypeptide 1 Kit FLISA, GHRH Kit FLISA, ADCYAP1 Kit FLISA, ghrh Kit FLISA, Ghrh Kit FLISA, LOC780526 Kit FLISA
- Pathways
- Hormone Transport, Hormone Activity, cAMP Metabolic Process
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