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BMP4 Kit ELISA

BMP4 Reactivité: Souris AA 293-408 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Osseous Tissue, Serum
N° du produit ABIN3044692
  • Antigène Voir toutes BMP4 Kits ELISA
    BMP4 (Bone Morphogenetic Protein 4 (BMP4))
    Épitope
    AA 293-408
    Reactivité
    • 10
    • 5
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 2
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    31.2-2000 pg/mL
    Seuil minimal de détection
    31.2 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse BMP-4
    Marque
    PicoKine™
    Type d'échantillon
    Osseous Tissue, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: CHO
    Immunogen sequence: S293-R408
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Homologie
    Bovine,Monkey
    Sensibilité
    <10pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Immunogen sequence: S293-R408
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Plaque
    Pre-coated
    Protocole
    mouse BMP-4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for BMP-4 has been precoated onto 96-well plates. Standards (Expression system for standard: CHO, Immunogen sequence: S293-R408) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BMP-4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse BMP-4 amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse BMP-4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse bone tissue, cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse BMP-4 standard solution and each sample be measured in duplicate.

    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Chen, Yang, Wu, Li, Li, Chang, Dai, Wang, Chang: "IL-33/ST2 axis mediates hyperplasia of intrarenal urothelium in obstructive renal injury." dans: Experimental & molecular medicine, Vol. 50, Issue 4, pp. 36, (2018) (PubMed).

  • Antigène Voir toutes BMP4 Kits ELISA
    BMP4 (Bone Morphogenetic Protein 4 (BMP4))
    Autre désignation
    BMP4 (BMP4 Produits)
    Synonymes
    BMP2B Kit ELISA, BMP2B1 Kit ELISA, MCOPS6 Kit ELISA, OFC11 Kit ELISA, ZYME Kit ELISA, Bmp-4 Kit ELISA, Bmp2b Kit ELISA, Bmp2b-1 Kit ELISA, Bmp2b1 Kit ELISA, BOMPR4A Kit ELISA, bmp-4 Kit ELISA, zbmp-4 Kit ELISA, zgc:100779 Kit ELISA, BMP-4 Kit ELISA, XBMP-4 Kit ELISA, bmp2b Kit ELISA, bmp2b1 Kit ELISA, bmp4 Kit ELISA, ofc11 Kit ELISA, xbmp4 Kit ELISA, zyme Kit ELISA, BMP4 Kit ELISA, bone morphogenetic protein 4 Kit ELISA, bone morphogenetic protein 4 L homeolog Kit ELISA, bone morphogenetic protein 4 S homeolog Kit ELISA, BMP4 Kit ELISA, Bmp4 Kit ELISA, bmp4 Kit ELISA, bmp4.L Kit ELISA, bmp4.S Kit ELISA
    Sujet

    Protein Function: Induces cartilage and bone formation. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction. .

    Background: Bone morphogenetic protein 4 is a protein that in humans is encoded by the BMP4 gene which is located to 14q22-q23. The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. BMP4 is a polypeptide belonging to the TGF-i² superfamily of proteins. It, like other bone morphogenetic proteins, is involved in bone and cartilage development, specifically tooth and limb development and fracture repair. It has been shown to be involved in muscle development, bone mineralization, and uteric bud development. BMP4 has also been implicated in Fibrodysplasia Ossificans Progressiva in which it is underexpressed. In human embryonic development, BMP4 is a critical signaling molecule required for the early differentiation of the embryo and establishing of a dorsal-ventral axis. BMP4 is secreted from the dorsal portion of the notochord, and it acts in concert with sonic hedgehog (released from the ventral portion of the notochord) to establish a dorsal-ventral axis for the differentiation of later structures. Also, BMP4 stimulates differentiation of overlying ectodermal tissue. Inhibition of the BMP4 signal (by chordin, noggin, or follistatin) causes the ectoderm to differentiate into the neural plate.

    Synonyms: Bone morphogenetic protein 4,BMP-4,Bone morphogenetic protein 2B,BMP-2B,Bmp4,Bmp-4, Dvr-4,

    Full Gene Name: Bone morphogenetic protein 4

    Cellular Localisation: Secreted, extracellular space, extracellular matrix.
    ID gène
    12159
    UniProt
    P21275
    Pathways
    Steroid Hormone Mediated Signaling Pathway, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
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