DKK1 Kit ELISA

N° du produit ABIN3044704

Détail du produit DKK1 Kit ELISA

Antigène: DKK1 (Dickkopf Homolog 1 (DKK1))

Épitope: AA 23-270

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 46.9-3000 pg/mL

Seuil minimal de détection: 46.9 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat DKK1

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: S23-H270

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <15pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Immunogen sequence: S23-H270

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plaque: Pre-coated

Protocole: rat DKK1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for DKK1 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: S23-H270) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for DKK1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat DKK1 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 3000pg/mL, 1500pg/mL, 750pg/mL, 375.pg/mL, 187.5pg/mL, 93.8pg/mL, 46.9pg/mL rat DKK1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernatants, serum or plasma (heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each rat DKK1 standard solution and each sample is measured in duplicate.

Restrictions: For Research Use only

Stockage

Buffer: heparin or EDTA

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: DKK1 (Dickkopf Homolog 1 (DKK1))

Autre désignation: DKK1 (DKK1 Produits)

Synonymes: DKK-1 Kit ELISA, SK Kit ELISA, AI414495 Kit ELISA, SGY Kit ELISA, SGY-1 Kit ELISA, SGY1 Kit ELISA, Soggy Kit ELISA, mSgy Kit ELISA, dkk1 Kit ELISA, dkk Kit ELISA, dkk-1 Kit ELISA, xdkk1 Kit ELISA, Xdkk-1 Kit ELISA, Dickkopf1 Kit ELISA, dickkopf-1 Kit ELISA, mdkk-1 Kit ELISA, dkk1-A Kit ELISA, dickkopf WNT signaling pathway inhibitor 1 Kit ELISA, dickkopf like acrosomal protein 1 Kit ELISA, dickkopf-related protein 1 Kit ELISA, dickkopf-like 1 Kit ELISA, dickkopf WNT signaling pathway inhibitor 1b Kit ELISA, dickkopf protein Kit ELISA, dickkopf WNT signaling pathway inhibitor 1 S homeolog Kit ELISA, DKK1 Kit ELISA, Dkk1 Kit ELISA, DKKL1 Kit ELISA, LOC100228789 Kit ELISA, Dkkl1 Kit ELISA, dkk1b Kit ELISA, dkk1 Kit ELISA, dickkopf Kit ELISA, dkk1.S Kit ELISA

Sujet:

Background: Dickkopf-related protein 1 is a protein that in humans is encoded by the DKK1 gene, which was mapped to chromosome 10q11.2. This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow, plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.

Synonyms: Dickkopf homolog 1 (Xenopus laevis) (Predicted) ,Dickkopf-like protein 1 ,Protein Dkk1 ,Dkk1 ,DKK1 , Dkk1_predicted , LOC100912050 ,rCG_48380 ,

Full Gene Name: dickkopf WNT signaling pathway inhibitor 1

ID gène: 293897

UniProt: D3Z9J1

Pathways: Signalisation WNT, Regulation of Muscle Cell Differentiation, Positive Regulation of fat Cell Differentiation