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INHBA Kit ELISA

INHBA Reactivité: Souris AA 311-426 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Saliva, Serum
N° du produit ABIN411255
  • Antigène Voir toutes INHBA Kits ELISA
    INHBA (Inhibin, beta A (INHBA))
    Épitope
    AA 311-426
    Reactivité
    • 6
    • 5
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    15.6-1000 pg/mL
    Seuil minimal de détection
    15.6 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Activin A
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Saliva
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: CHO
    Immunogen sequence: G311-S426
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Homologie
    Bovine,Monkey,Rabbit
    Sensibilité
    <10pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: CHO
    Immunogen sequence: G311-S426
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Tissue Specificity: Uterus, ovary and liver.

    Plaque
    Pre-coated
    Protocole
    mouse Activin A ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Activin A has been precoated onto 96-well plates. Standards(CHO, G311-S426) and test samples are added to the wells, a biotinylated detection monoclonal antibody from mouse specific for Activin A is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Activin A amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse Activin A standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum, plasma(heparin, EDTA) or saliva to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse Activin A standard solution and each sample be measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(pg/ml): 95, Standard deviation: 4.8, CV(%): 5
    • Sample 2: n=16, Mean(pg/ml): 289, Standard deviation: 15.0, CV(%): 5.2
    • Sample 3: n=16, Mean(pg/ml): 453, Standard deviation: 23.6, CV(%): 5.2,
    • Sample 1: n=24, Mean(pg/ml): 98, Standard deviation: 7.8, CV(%): 8
    • Sample 2: n=24, Mean(pg/ml): 301, Standard deviation: 18.7, CV(%): 6.2
    • Sample 3: n=24, Mean(pg/ml): 495, Standard deviation: 24.3, CV(%): 4.9
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Zhang, Ju, Nie, Song, Xu, Gao: "Adenovirus‑mediated knockdown of activin A receptor type 2A attenuates immune‑induced hepatic fibrosis in mice and inhibits interleukin‑17‑induced activation of primary hepatic stellate cells." dans: International journal of molecular medicine, Vol. 42, Issue 1, pp. 279-289, (2018) (PubMed).

  • Antigène Voir toutes INHBA Kits ELISA
    INHBA (Inhibin, beta A (INHBA))
    Autre désignation
    INHBA (INHBA Produits)
    Synonymes
    EDF Kit ELISA, FRP Kit ELISA, actba Kit ELISA, inhba Kit ELISA, inhbaa-001 Kit ELISA, edf Kit ELISA, frp Kit ELISA, INHB Kit ELISA, inhbal Kit ELISA, zgc:158348 Kit ELISA, inhibin beta A subunit Kit ELISA, inhibin beta-A Kit ELISA, inhibin, beta Aa Kit ELISA, inhibin beta A Kit ELISA, inhibin, beta Ab Kit ELISA, INHBA Kit ELISA, Inhba Kit ELISA, inhbaa Kit ELISA, inhba Kit ELISA, inhbab Kit ELISA
    Sujet

    Protein Function: Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.

    Background: Activin A is a homodimer of 14 kDa beta-A. Activin A, a cytokine member of the transforming growth factor-beta superfamily, is expressed locally by the mesenchymal component of the hemopoietic microenvironment. Its expression is regulated on the mRNA level by different cytokines, and the biological activity of the protein is tightly controlled by several inhibitory molecules. Inhibins and activins are members of the transforming growth factor beta superfamily and are known to modulate the growth and differentiation of several cell types. Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. The standard product used in this kit is recombinant Activin A, which is composed of two single chains of 116 amino acids with the molecular mass of 26KDa.

    Synonyms: Inhibin beta A chain,Activin beta-A chain,Inhba,

    Full Gene Name: Inhibin beta A chain

    Cellular Localisation: Secreted.
    ID gène
    16323
    UniProt
    Q04998
    Pathways
    Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Autophagy
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