EGF Kit ELISA

N° du produit ABIN411264

Détail du produit EGF Kit ELISA

Antigène: EGF (Epidermal Growth Factor (EGF))

Épitope: AA 971-1023

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 4.7-300 pg/mL

Seuil minimal de détection: 4.7 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human EGF

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate), Urine

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli
Immunogen sequence: N971-R1023

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <1pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: E.coli
Immunogen sequence: N971-R1023

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Contains 9 EGF-like domains.

Tissue Specificity: Expressed in kidney, salivary gland, cerebrum and prostate. .

Plaque: Pre-coated

Protocole: human EGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for EGF has been precoated onto 96-well plates. Standards(E.coli, N971-R1023) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for EGF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human EGF amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 300pg/mL, 150pg/mL, 75pg/mL, 37.5pg/mL, 18.8pg/mL, 9.4pg/mL, 4.7pg/mL human EGF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma( heparin, EDTA, citrate) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human EGF standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 42.1, Standard deviation: 1.98, CV(%): 4.7
• Sample 2: n=16, Mean(pg/ml): 105, Standard deviation: 5.67, CV(%): 5.4
• Sample 3: n=16, Mean(pg/ml): 197, Standard deviation: 6.30, CV(%): 3.2,
• Sample 1: n=24, Mean(pg/ml): 45.2, Standard deviation: 3.21, CV(%): 7.1
• Sample 2: n=24, Mean(pg/ml): 112, Standard deviation: 5.71, CV(%): 5.1
• Sample 3: n=24, Mean(pg/ml): 227, Standard deviation: 13.17, CV(%): 5.8

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour EGF Kit ELISA (ABIN411264)

Russo, Linsalata, Clemente, DAttoma, Orlando, Campanella, Giotta, Riezzo: "The effects of fluorouracil, epirubicin, and cyclophosphamide (FEC60) on the intestinal barrier function and gut peptides in breast cancer patients: an observational study." dans: BMC cancer, Vol. 13, pp. 56, (2013) (PubMed).

Tabeefar, Beigmohammadi, Javadi, Abdollahi, Mahmoodpoor, Ahmadi, Honarmand, Najafi, Mojtahedzadeh: "Effects of Pantoprazole on Systemic and Gastric Pro- and Anti-inflammatory Cytokines in Critically Ill Patients." dans: Iranian journal of pharmaceutical research : IJPR, Vol. 11, Issue 4, pp. 1051-8, (2013) (PubMed).

Ge, Yu, Petitte, Zhang: "Epidermal growth factor-induced proliferation of chicken primordial germ cells: involvement of calcium/protein kinase C and NFKB1." dans: Biology of reproduction, Vol. 80, Issue 3, pp. 528-36, (2009) (PubMed).

Détail du antigène

Antigène: EGF (Epidermal Growth Factor (EGF))

Autre désignation: EGF (EGF Produits)

Synonymes: HOMG4 Kit ELISA, URG Kit ELISA, AI790464 Kit ELISA, CEGF Kit ELISA, epidermal growth factor Kit ELISA, pro-epidermal growth factor Kit ELISA, EGF Kit ELISA, egf Kit ELISA, CpipJ_CPIJ020278 Kit ELISA, Egf Kit ELISA

Sujet:

Protein Function: EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941). .

Background: Epidermal growth factor or EGF is a growth factor that plays an important role in the regulation of cell growth, proliferation, and differentiation by binding to its receptor EGFR. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bonds. EGF acts by binding with high affinity to epidermal growth factor receptor(EGFR) on the cell surface and stimulating the intrinsic protein-tyrosine kinase activity of the receptor. EGF results in cellular proliferation, differentiation, and survival. It also has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. EGF has strong expression in kidney, salivary gland, cerebrum, and prostate, moderate expression in trachea and thyroid, and low expression in bone marrow, heart, spleen, thymus, uterus, and colon. No expression was detected in adrenal gland, liver, lung, cerebellum, placenta, and small intestine.

Synonyms: Pro-epidermal growth factor,EGF,Epidermal growth factor,Urogastrone,EGF,

Full Gene Name: Pro-epidermal growth factor

Cellular Localisation: Membrane, Single-pass type I membrane protein.

ID gène: 1950

UniProt: E7EVD2

Pathways: Signalisation NF-kappaB, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation