IL-2 Kit ELISA

N° du produit ABIN411296

Détail du produit IL-2 Kit ELISA

Antigène: IL-2 (IL2) (Interleukin 2 (IL2))

Épitope: AA 21-169

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6-1000 pg/mL

Seuil minimal de détection: 15.6 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IL-2

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli,A21-Q169

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <1pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: E.coli
Immunogen sequence: A21-Q169

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plaque: Pre-coated

Protocole: mouse IL-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-2 has been precoated onto 96-well plates. Standards (E.coli,A21-Q169) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-2 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse IL-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma( heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse IL-2 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 81, Standard deviation: 4.536, CV(%): 5.6
• Sample 2: n=16, Mean(pg/ml): 215, Standard deviation: 11.61, CV(%): 5.4
• Sample 3: n=16, Mean(pg/ml): 590, Standard deviation: 25.37, CV(%): 4.3,
• Sample 1: n=24, Mean(pg/ml): 86, Standard deviation: 5.246, CV(%): 6.1
• Sample 2: n=24, Mean(pg/ml): 193, Standard deviation: 13.32, CV(%): 6.9
• Sample 3: n=24, Mean(pg/ml): 575, Standard deviation: 29.9, CV(%): 5.2

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour IL-2 Kit ELISA (ABIN411296)

Shen, Zhang, Li, Liu, Yu, Hu, Hui, Guo, Zhang: "Oroxylin A inhibits the generation of Tregs in non-small cell lung cancer." dans: Oncotarget, Vol. 8, Issue 30, pp. 49395-49408, (2018) (PubMed).

Dong, Han, Wang, Qin, Yang, Cao, Chen: "Role of serotonin on the intestinal mucosal immune response to stress-induced diarrhea in weaning mice." dans: BMC gastroenterology, Vol. 17, Issue 1, pp. 82, (2018) (PubMed).

Liang, Mao, Sun, Li, Li, Yu, Ma, Gu, Zhang, Taniguchi, Li: "Core Fucosylation of the T Cell Receptor Is Required for T Cell Activation." dans: Frontiers in immunology, Vol. 9, pp. 78, (2018) (PubMed).

Zhang, Li, Wang, Xie, Li, Zuo, Kong, Wang, Wang: "Immune Protection of Rhoptry Protein 21 (ROP21) of Toxoplasma gondii as a DNA Vaccine Against Toxoplasmosis." dans: Frontiers in microbiology, Vol. 9, pp. 909, (2018) (PubMed).

Wang, Liu, Chen, Chen: "Physicochemical characterization of the oolong tea polysaccharides with high molecular weight and their synergistic effects in combination with polyphenols on hepatocellular carcinoma." dans: Biomedicine & pharmacotherapy, Vol. 90, pp. 160-170, (2017) (PubMed).

Yang, Shi, Wumaierniyazi, Shan, Miao, Wu, Zhou, Yusup, Upur, Aikemu: "Enhanced antitumor efficacy and reduced toxicity of Abnormal Savda Munziq on tumor bearing mice treated with chemotherapy." dans: Oncotarget, Vol. 8, Issue 54, pp. 92682-92698, (2017) (PubMed).

Wang, Su, Dong, Shi, Gao, Wang: "Hybrid phage displaying SLAQVKYTSASSI induces protection against Candida albicans challenge in BALB/c mice." dans: Human vaccines & immunotherapeutics, Vol. 10, Issue 4, pp. 1057-63, (2016) (PubMed).

Chen, Zhang, Sun: "Immunological adjuvant effect of the peptide fraction from the larvae of Musca domestica." dans: BMC complementary and alternative medicine, Vol. 15, Issue 1, pp. 427, (2016) (PubMed).

Huai, Dong, Zhu, Li, Cao, Gao, Yang, Wang, Mao: "Genetically Engineered Virus Nanofibers as an Efficient Vaccine for Preventing Fungal Infection." dans: Advanced healthcare materials, Vol. 5, Issue 7, pp. 786-94, (2016) (PubMed).

Xie, Cao, Zhao, Yu, Qi, Dai: "IDO expressing dendritic cells suppress allograft rejection of small bowel transplantation in mice by expansion of Foxp3+ regulatory T cells." dans: Transplant immunology, Vol. 33, Issue 2, pp. 69-77, (2015) (PubMed).

Hu, Jing, Wang, Zhang, Yong, Wang: "The positive effects of Ginsenoside Rg1 upon the hematopoietic microenvironment in a D-Galactose-induced aged rat model." dans: BMC complementary and alternative medicine, Vol. 15, pp. 119, (2015) (PubMed).

Liang, Liu, Chen, Sun: "Antitumor activity of the protein and small molecule component fractions from Agrocybe aegerita through enhancement of cytokine production." dans: Journal of medicinal food, Vol. 17, Issue 4, pp. 439-46, (2014) (PubMed).

Yu, Liu, Chen, Zeng: "Anti-inflammatory effect of Qingwen Baidu Decoction (?????) in sepsis rats." dans: Chinese journal of integrative medicine, Vol. 20, Issue 12, pp. 934-43, (2014) (PubMed).

Eker, Yavasci, Cangur, Kirli, Sarandol: "Can BDNF and IL-2 be indicators for the diagnosis in schizophrenic patients with depressive symptoms?" dans: Acta neuropsychiatrica, Vol. 26, Issue 5, pp. 291-7, (2014) (PubMed).

Cheng, Wu, Gao, Xu: "Analysis of influence of baicalin joint resveratrol retention enema on the TNF-?, SIgA, IL-2, IFN-? of rats with respiratory syncytial virus infection." dans: Cell biochemistry and biophysics, Vol. 70, Issue 2, pp. 1305-9, (2014) (PubMed).

Meng, Ning, Qi, He, Jie, Lin, Huang, Li, Li: "Structure and antitumor and immunomodulatory activities of a water-soluble polysaccharide from Dimocarpus longan pulp." dans: International journal of molecular sciences, Vol. 15, Issue 3, pp. 5140-62, (2014) (PubMed).

Liu, Shan, Dong, Liu, Ma, Liu: "Combined early fluid resuscitation and hydrogen inhalation attenuates lung and intestine injury." dans: World journal of gastroenterology, Vol. 19, Issue 4, pp. 492-502, (2013) (PubMed).

Zhong, Cheng, Long, He, Qi, Xiang, Zhao, Zhu: "Dynamically expressed microRNA-15b modulates the activities of CD8+ T lymphocytes in mice with Lewis lung carcinoma." dans: Journal of translational medicine, Vol. 11, pp. 71, (2013) (PubMed).

Sofian, Aghakhani, Farazi, Banifazl, Eslamifar, Rashidi, Khadem Sadegh, Ramezani: "Serum profile of T helper 1 and T helper 2 cytokines in hepatitis C virus infected patients." dans: Hepatitis monthly, Vol. 12, Issue 12, pp. e6156, (2013) (PubMed).

Chen, Ni, Ye, Sun, Li, Xu: "Stephanthraniline A inhibits the proliferation and activation of T cells in vitro and in vivo." dans: European journal of pharmacology, Vol. 685, Issue 1-3, pp. 186-97, (2012) (PubMed).

Détail du antigène

Antigène: IL-2 (IL2) (Interleukin 2 (IL2))

Autre désignation: IL2 (IL2 Produits)

Synonymes: IL2 Kit ELISA, IL-2 Kit ELISA, il2 Kit ELISA, TCGF Kit ELISA, lymphokine Kit ELISA, Il-2 Kit ELISA, POIL2 Kit ELISA, interleukin 2 Kit ELISA, interleukin-15 Kit ELISA, IL2 Kit ELISA, il2 Kit ELISA, LOC100303688 Kit ELISA, Il2 Kit ELISA

Sujet:

Protein Function: Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine- activated killer cells, natural killer cells, and glioma cells.

Background: Interleukin-2(IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells. It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines. The lymphokine interleukin-2(IL-2) is responsible for autocrine cell cycle progression and regulation of immune responses. IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression. IL-2 can act as a growth hormone for both B and T lymphocytes. The human gene for interleukin 2(IL2) is assigned to chromosome 4. Mouse IL-2 contains 149 amino acids in mature form with the molecular mass of 17.2KDa.

Synonyms: Interleukin-2,IL-2,T-cell growth factor,TCGF,Il2,Il-2,

Full Gene Name: Interleukin-2

Cellular Localisation: Secreted.

ID gène: 16183

UniProt: P04351

Pathways: Signalistation JAK/STAT, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation