IL-4 Kit ELISA

N° du produit ABIN411299

Détail du produit IL-4 Kit ELISA

Antigène: IL-4 (IL4) (Interleukin 4 (IL4))

Épitope: AA 25-153

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6-1000 pg/mL

Seuil minimal de détection: 15.6 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-4

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli
Immunogen sequence: H25-S153

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <1.5pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: E.coli
Immunogen sequence: H25-S153

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plaque: Pre-coated

Protocole: human IL-4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-4 has been precoated onto 96-well plates. Standards(E.coli, H25-S153) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-4 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human IL-4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IL-4 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 55.4, Standard deviation: 2.5, CV(%): 4.5
• Sample 2: n=16, Mean(pg/ml): 361, Standard deviation: 14.8, CV(%): 4.1
• Sample 3: n=16, Mean(pg/ml): 638, Standard deviation: 37.64, CV(%): 5.9,
• Sample 1: n=24, Mean(pg/ml): 45.8, Standard deviation: 2.977, CV(%): 6.5
• Sample 2: n=24, Mean(pg/ml): 375, Standard deviation: 24, CV(%): 6.4
• Sample 3: n=24, Mean(pg/ml): 656, Standard deviation: 46.58, CV(%): 7.1

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour IL-4 Kit ELISA (ABIN411299)

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Jin, Wu, Wang, Peng: "Capsaicin induces immunogenic cell death in human osteosarcoma cells." dans: Experimental and therapeutic medicine, Vol. 12, Issue 2, pp. 765-770, (2016) (PubMed).

Li, Hu, Wang, Wu, Zhang: "Different profiles of notch signaling in cigarette smoke-induced pulmonary emphysema and bleomycin-induced pulmonary fibrosis." dans: Inflammation research : official journal of the European Histamine Research Society ... [et al.], Vol. 64, Issue 5, pp. 363-71, (2015) (PubMed).

Huang, Wang, Li, Ge, Chen, Chen: "Trichostatin A reduces cisplatin-induced ototoxicity through the STAT6 signaling pathway." dans: International journal of molecular medicine, Vol. 36, Issue 2, pp. 493-500, (2015) (PubMed).

Presti, DOrazio, Labra, La Ferla, Mezzasalma, Bizzaro, Giardina, Michelotti, Tursi, Vassallo, Di Gennaro: "Evaluation of the probiotic properties of new Lactobacillus and Bifidobacterium strains and their in vitro effect." dans: Applied microbiology and biotechnology, Vol. 99, Issue 13, pp. 5613-26, (2015) (PubMed).

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Berni Canani, Paparo, Nocerino, Cosenza, Pezzella, Di Costanzo, Capasso, Del Monaco, DArgenio, Greco, Salvatore: "Differences in DNA methylation profile of Th1 and Th2 cytokine genes are associated with tolerance acquisition in children with IgE-mediated cow's milk allergy." dans: Clinical epigenetics, Vol. 7, Issue 1, pp. 38, (2015) (PubMed).

Jin, Cheng, Liu, Wu, Wang, Wei, Zhou, Qin, Jia, Zhen et al.: "Improvement of functional recovery by chronic metformin treatment is associated with enhanced alternative activation of microglia/macrophages and increased angiogenesis and neurogenesis following ..." dans: Brain, behavior, and immunity, Vol. 40, pp. 131-42, (2014) (PubMed).

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Li, Li, Zuo, Zhou, Hao, Dang, Jiang, He, Deng: "Up-regulated expression of Tim-3/Gal-9 at maternal-fetal interface in pregnant woman with recurrent spontaneous abortion." dans: Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban, Vol. 34, Issue 4, pp. 586-90, (2014) (PubMed).

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Li, Yu, Ying, Yu: "Antiviral effects of modified dingchuan decoction against respiratory syncytial virus infection in vitro and in an immunosuppressive mouse model." dans: Journal of ethnopharmacology, Vol. 147, Issue 1, pp. 238-44, (2013) (PubMed).

Sofian, Aghakhani, Farazi, Banifazl, Eslamifar, Rashidi, Khadem Sadegh, Ramezani: "Serum profile of T helper 1 and T helper 2 cytokines in hepatitis C virus infected patients." dans: Hepatitis monthly, Vol. 12, Issue 12, pp. e6156, (2013) (PubMed).

Zhu, Qiu, Liu, Dong, Weng, Hu, Kuang, Gao, Dan, Tao: "Tolerogenic dendritic cells generated by RelB silencing using shRNA prevent acute rejection." dans: Cellular immunology, Vol. 274, Issue 1-2, pp. 12-8, (2012) (PubMed).

Ozkara, Keles, Ilhan, Gungor, Kaygusuz, Alpay: "The relationship between Th1/Th2 balance and 1?,25-dihydroxyvitamin D? in patients with nasal polyposis." dans: European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery, Vol. 269, Issue 12, pp. 2519-24, (2012) (PubMed).

Détail du antigène

Antigène: IL-4 (IL4) (Interleukin 4 (IL4))

Autre désignation: IL4 (IL4 Produits)

Synonymes: IL4 Kit ELISA, IL-4 Kit ELISA, BCGF-1 Kit ELISA, BCGF1 Kit ELISA, BSF-1 Kit ELISA, BSF1 Kit ELISA, Il-4 Kit ELISA, Il4e12 Kit ELISA, IL2 Kit ELISA, interleukin 4 Kit ELISA, IL4 Kit ELISA, Il4 Kit ELISA

Sujet:

Protein Function: Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. .

Background: Interleukin-4(IL-4), also knowns as a B-cell stimulatory factor1(BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells.The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-directed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis. The mouse and human genes and their protein products show structural and functional similarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5. The standard product used in this kit is recombinant human IL-4, consisting of 130 amino acids with the molecular mass of 14KDa.

Synonyms: Interleukin-4 ,IL-4 ,B-cell stimulatory factor 1 ,Lymphocyte stimulatory factor 1 ,IL4 ,hCG_24113 ,

Full Gene Name: Interleukin-4

Cellular Localisation: Secreted.

ID gène: 3565

UniProt: D4HNR6

Pathways: Signalistation JAK/STAT, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation