IL-6 Kit ELISA

N° du produit ABIN411304

Détail du produit IL-6 Kit ELISA

Antigène: IL-6 (IL6) (Interleukin 6 (IL6))

Épitope: AA 25-211

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6-1000 pg/mL

Seuil minimal de détection: 15.6 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IL-6

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli
Immunogen sequence: F25-T211

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <1pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: E.coli
Immunogen sequence: F25-T211

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plaque: Pre-coated

Protocole: mouse IL-6 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-6 has been precoated onto 96-well plates. Standards(E.coli, F25-T211) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-6 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-6 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse IL-6 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse IL-6 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 97.5, Standard deviation: 6.24, CV(%): 6.4
• Sample 2: n=16, Mean(pg/ml): 359, Standard deviation: 16.16, CV(%): 4.5
• Sample 3: n=16, Mean(pg/ml): 654, Standard deviation: 31.39, CV(%): 4.8,
• Sample 1: n=24, Mean(pg/ml): 102, Standard deviation: 7.65, CV(%): 7.5
• Sample 2: n=24, Mean(pg/ml): 437, Standard deviation: 25.78, CV(%): 5.9
• Sample 3: n=24, Mean(pg/ml): 716, Standard deviation: 37.95, CV(%): 5.3

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour IL-6 Kit ELISA (ABIN411304)

Cai, Chen, Zhong, Zhang, Jiang, Xu, Shi: "Synergistic Effect of Compounds from a Chinese Herb: Compatibility and Dose Optimization of Compounds from N-Butanol Extract of Ipomoea stolonifera." dans: Scientific reports, Vol. 6, pp. 27014, (2018) (PubMed).

Liu, Xu, Chang, Qin, Yin, Yang: "4,4'-diaponeurosporene, a C30 carotenoid, effectively activates dendritic cells via CD36 and NF-κB signaling in a ROS independent manner." dans: Oncotarget, Vol. 7, Issue 27, pp. 40978-40991, (2018) (PubMed).

Lin, Chen, Xia, Yang: "MiR674 inhibits the neuraminidase-stimulated immune response on dendritic cells via down-regulated Mbnl3." dans: Oncotarget, Vol. 7, Issue 31, pp. 48978-48994, (2018) (PubMed).

Li, Jin, Wang, Li, Liu, Chang, Wang, Liu: "Live attenuated Salmonella displaying HIV-1 10E8 epitope on fimbriae: systemic and mucosal immune responses in BALB/c mice by mucosal administration." dans: Scientific reports, Vol. 6, pp. 29556, (2018) (PubMed).

Pang, Wu, Peng, Yang, Kuai, Qin, Cao, Sun, Chen, Vitek, Jiang: "Potential implications of Apolipoprotein E in early brain injury after experimental subarachnoid hemorrhage: Involvement in the modulation of blood-brain barrier integrity." dans: Oncotarget, Vol. 7, Issue 35, pp. 56030-56044, (2018) (PubMed).

Dong, Liu, Meng, Liu, Bi, Wu, Jin, Yao, Tang, Wang, Li, Zhang, Yu, Zhan, Chen, Ge, Yang, Li: "Keratin 8 limits TLR-triggered inflammatory responses through inhibiting TRAF6 polyubiquitination." dans: Scientific reports, Vol. 6, pp. 32710, (2018) (PubMed).

Gao, Huang, Zhao, Hu, Li, Guo, Zhao, Lu: "LL202 protects against dextran sulfate sodium-induced experimental colitis in mice by inhibiting MAPK/AP-1 signaling." dans: Oncotarget, Vol. 7, Issue 39, pp. 63981-63994, (2018) (PubMed).

Yang, Lai, Zheng, Kang, Xian, Wang, Shi, Meng, Li, Chen, Zhang: "A novel pH-controlled hydrogen sulfide donor protects gastric mucosa from aspirin-induced injury." dans: Journal of cellular and molecular medicine, Vol. 21, Issue 10, pp. 2441-2451, (2018) (PubMed).

Wang, Zhang, Yang, Zou, Wang, Liu, Liu, Zhang, Xiao, Wang: "Resveratrol protects against L-arginine-induced acute necrotizing pancreatitis in mice by enhancing SIRT1-mediated deacetylation of p53 and heat shock factor 1." dans: International journal of molecular medicine, Vol. 40, Issue 2, pp. 427-437, (2018) (PubMed).

Zhang, Wu, Xu, He, Yang, Li, Li, Ao, Cheng, Jia: "The cystathionine β-synthase/hydrogen sulfide pathway contributes to microglia-mediated neuroinflammation following cerebral ischemia." dans: Brain, behavior, and immunity, Vol. 66, pp. 332-346, (2018) (PubMed).

Li, Diao, Guo, Huang, Yang, Feng, Yan, Ning, Zheng, Chen, Wu: "VSIG4 inhibits proinflammatory macrophage activation by reprogramming mitochondrial pyruvate metabolism." dans: Nature communications, Vol. 8, Issue 1, pp. 1322, (2018) (PubMed).

Du, Chen, Wang, Sun: "Pathway analysis of global gene expression change in dendritic cells induced by the polysaccharide from the roots of Actinidia eriantha." dans: Journal of ethnopharmacology, Vol. 214, pp. 141-152, (2018) (PubMed).

Wang, Chen, Ni, Cheng, Jia, Zhen: "miRNA-3473b contributes to neuroinflammation following cerebral ischemia." dans: Cell death & disease, Vol. 9, Issue 1, pp. 11, (2018) (PubMed).

Zhang, Feng, Cui, Yang, Zhu et al.: "Pre-B cell leukemia transcription factor 3 induces inflammatory responses in human umbilical vein endothelial cells and murine sepsis via acting a competing endogenous RNA for high mobility group box ..." dans: Molecular medicine reports, Vol. 17, Issue 4, pp. 5805-5813, (2018) (PubMed).

Lu, Zhang, Zhang, Zhou, Gao, Liu, Wang, Wu, Li, Hang: "Peroxiredoxin 2 activates microglia by interacting with Toll-like receptor 4 after subarachnoid hemorrhage." dans: Journal of neuroinflammation, Vol. 15, Issue 1, pp. 87, (2018) (PubMed).

Zhu, Li, Zhang, Ding, Zhu, Huang, Zhang, Jia, Li: "COX-2 contributes to LPS-induced Stat3 activation and IL-6 production in microglial cells." dans: American journal of translational research, Vol. 10, Issue 3, pp. 966-974, (2018) (PubMed).

Xu, Liu, Wang, Li, Wu, Li, Di, You, Jiang et al.: "3-(1H-Benzo[d]imidazol-6-yl)-5-(4-fluorophenyl)-1,2,4-oxadiazole (DDO7232), a Novel Potent Nrf2/ARE Inducer, Ameliorates DSS-Induced Murine Colitis and Protects NCM460 Cells against Oxidative Stress ..." dans: Oxidative medicine and cellular longevity, Vol. 2018, pp. 3271617, (2018) (PubMed).

Li, Fan, Xia, Xiao, Wang, Sun, Zhang, He: "The immunosuppressive characteristics of FB1 by inhibition of maturation and function of BMDCs." dans: International immunopharmacology, Vol. 47, pp. 206-211, (2017) (PubMed).

Bai, Yin, Feng, Cao, Wu, Zhu, Li, Tu, Chai: "Corydalis hendersonii Hemsl. protects against myocardial injury by attenuating inflammation and fibrosis via NF-κB and JAK2-STAT3 signaling pathways." dans: Journal of ethnopharmacology, Vol. 207, pp. 174-183, (2017) (PubMed).

Qu, Zhang, Cai, Li, Ma, Xu, Lu: "Exosomes derived from miR-181-5p-modified adipose-derived mesenchymal stem cells prevent liver fibrosis via autophagy activation." dans: Journal of cellular and molecular medicine, (2017) (PubMed).

Détail du antigène

Antigène: IL-6 (IL6) (Interleukin 6 (IL6))

Autre désignation: IL6 (IL6 Produits)

Synonymes: BSF2 Kit ELISA, HGF Kit ELISA, HSF Kit ELISA, IFNB2 Kit ELISA, IL-6 Kit ELISA, Il-6 Kit ELISA, ILg6 Kit ELISA, Ifnb2 Kit ELISA, il6 Kit ELISA, CHIL-6 Kit ELISA, interleukin 6 Kit ELISA, interleukin-6 Kit ELISA, IL6 Kit ELISA, Il6 Kit ELISA, il-6 Kit ELISA, IL-6 Kit ELISA

Sujet:

Background: The human interferon-beta 2 gene(IFNB2) product is identical to that for the B-cell stimulation factor-2(BSF-2), the hybridoma growth factor(HGF)("interleukin-6"), and the hepatocyte stimulating factor(HSF). Proteins derived from this gene mediate the plasma protein response to tissue injury(acute-phase response) and regulate the growth and differentiation of both B and T cells. Interleukin-6(IL6) has come to be regarded as a potential osteoporotic factor because it has stimulatory effects on cells of the osteoclast lineage, and, thus, may play a role in the pathogenesis of bone loss associated with estrogen deficiency. IL-6 has many roles essential to the regulation of the immune response, hematopoiesis, and bone resorption. It is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion. Overproduction of IL-6, a proinflammatory cytokine, is associated with a spectrum of age-related conditions including cardiovascular disease, osteoporosis, arthritis, type 2 diabetes, certain cancers, periodontal disease, frailty, and functional decline. BSF-2 is a novel interleukin consisting of 184 amino acids. The standard product used in this kit is recombinant IL-6, consisting of 188 amino acids with the molecular mass of 21.8 kDa.

Synonyms: Interleukin 6 ,Interleukin 6, isoform CRA_b ,Il6 ,mCG_11634 ,

Full Gene Name: interleukin 6

ID gène: 16193

UniProt: A2RTD1

Pathways: Signalisation TLR, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome