IL-10 Kit ELISA

N° du produit ABIN411306

Détail du produit IL-10 Kit ELISA

Antigène: IL-10 (IL10) (Interleukin 10 (IL10))

Épitope: AA 19-178

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 7.8-500 pg/mL

Seuil minimal de détection: 7.8 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-10

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli
Immunogen sequence: S19-N178

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <0.5pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: E.coli
Immunogen sequence: S19-N178

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Belongs to the IL-10 family.

Tissue Specificity: Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.

Plaque: Pre-coated

Protocole: human IL-10 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-10 has been precoated onto 96-well plates. Standards(E.coli, S19-N178) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-10 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-10 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL human IL-10 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human IL-10 standard solution and each sample is measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 25, Standard deviation: 1.35, CV(%): 5.4
• Sample 2: n=16, Mean(pg/ml): 112, Standard deviation: 5.38, CV(%): 4.8
• Sample 3: n=16, Mean(pg/ml): 280, Standard deviation: 8.68, CV(%): 3.1,
• Sample 1: n=24, Mean(pg/ml): 34, Standard deviation: 2.55, CV(%): 7.5
• Sample 2: n=24, Mean(pg/ml): 184, Standard deviation: 14.54, CV(%): 7.9
• Sample 3: n=24, Mean(pg/ml): 312, Standard deviation: 20.9, CV(%): 6.7

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour IL-10 Kit ELISA (ABIN411306)

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Gheybi, Farrokhi, Ravanbod, Ostovar, Mehrzad, Nematollahi: "The correlation of CD19 + CD24 + CD38 + B cells and other clinicopathological variables with the proportion of circulating Tregs in breast cancer patients." dans: Breast cancer (Tokyo, Japan), (2017) (PubMed).

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Li, Tang, Shuai, Jiang, Liu, Wang, Yao, Nie, Xie: "Mannose Receptor Mediates the Immune Response to Ganoderma atrum Polysaccharides in Macrophages." dans: Journal of agricultural and food chemistry, Vol. 65, Issue 2, pp. 348-357, (2016) (PubMed).

Yao, Li, Wu, Zhang, Wang: "Inflammatory response of macrophages cultured with Helicobacter pylori strains was regulated by miR-155." dans: International journal of clinical and experimental pathology, Vol. 8, Issue 5, pp. 4545-54, (2016) (PubMed).

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Détail du antigène

Antigène: IL-10 (IL10) (Interleukin 10 (IL10))

Autre désignation: IL10 (IL10 Produits)

Synonymes: CSIF Kit ELISA, GVHDS Kit ELISA, IL-10 Kit ELISA, IL10A Kit ELISA, TGIF Kit ELISA, Il-10 Kit ELISA, IL10X Kit ELISA, interleukin 10 Kit ELISA, IL10 Kit ELISA, Il10 Kit ELISA

Sujet:

Protein Function: Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.

Background: Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production. IL-10 is an important cytokine with anti-inflammatory, anti-immune, and antifibrotic functions. It is also an important regulatory cytokine whose involvement extends into diverse areas of the human immune system. IL-10 is a recently described natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8. Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to the development of skin squamous cell carcinomas after renal transplantation.1 The interleukin-10 locus contributes to the heritability of psoriasis susceptibility. With regard to sudden infant death, IL-10 is of special interest. This is an immunoregulatory cytokine that plays an important role in the development of infectious disease The mIL-10 gene is mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to human chromosome 1. The standard product used in this kit is recombinant human IL-10, consisting of 160 amino acids with the molecular mass of 18.6KDa.

Synonyms: Interleukin-10,IL-10,Cytokine synthesis inhibitory factor,CSIF,IL10,

Full Gene Name: Interleukin-10

Cellular Localisation: Secreted.

ID gène: 3586

UniProt: P22301

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints