MMP2 Kit ELISA

N° du produit ABIN411328

Détail du produit MMP2 Kit ELISA

Antigène: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Épitope: AA 30-660

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156-10000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human MMP-2

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin)

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: A30-C660

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: A30-C660

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Belongs to the peptidase M10A family.

Tissue Specificity: Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate. .

Plaque: Pre-coated

Protocole: human MMP-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MMP-2 has been precoated onto 96-well plates. Standards(NSO, A30-C660) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MMP-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human MMP-2 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human MMP-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernatants, serum, or plasma (heparin) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human MMP-2 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 1526, Standard deviation: 74.8, CV(%): 4.9
• Sample 2: n=16, Mean(pg/ml): 3864, Standard deviation: 216.4, CV(%): 5.6
• Sample 3: n=16, Mean(pg/ml): 6733, Standard deviation: 444.4, CV(%): 6.6,
• Sample 1: n=24, Mean(pg/ml): 1705, Standard deviation: 97.2, CV(%): 5.7
• Sample 2: n=24, Mean(pg/ml): 4012, Standard deviation: 256.8, CV(%): 6.4
• Sample 3: n=24, Mean(pg/ml): 6928, Standard deviation: 512.7, CV(%): 7.4

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour MMP2 Kit ELISA (ABIN411328)

Matin, Nemati, Ghobadi, Alipanah-Moghadam, Rezagholizadeh: "The effect of conjugated linoleic acid on oxidative stress and matrix metalloproteinases 2 and 9 in patients with COPD." dans: International journal of chronic obstructive pulmonary disease, Vol. 13, pp. 1449-1454, (2018) (PubMed).

Nosratzehi, Alijani, Moodi: "Salivary MMP-1, MMP-2, MMP-3 and MMP-13 Levels in Patients with Oral Lichen Planus and Squamous Cell Carcinoma" dans: Asian Pacific journal of cancer prevention : APJCP, Vol. 18, Issue 7, pp. 1947-1951, (2017) (PubMed).

Hu, Ni, Cao, Zhang, Wu, Yin, Lang, Lu: "The Angiogenic Effect of microRNA-21 Targeting TIMP3 through the Regulation of MMP2 and MMP9." dans: PLoS ONE, Vol. 11, Issue 2, pp. e0149537, (2016) (PubMed).

Bi, Zeng, Zhao, Wei, Yu, Wang, Yu, Cao, Shan, Wei: "miR-181a Induces Macrophage Polarized to M2 Phenotype and Promotes M2 Macrophage-mediated Tumor Cell Metastasis by Targeting KLF6 and C/EBPα." dans: Molecular therapy. Nucleic acids, Vol. 5, Issue 9, pp. e368, (2016) (PubMed).

Simmers, Gishto, Vyavahare, Kothapalli: "Nitric oxide stimulates matrix synthesis and deposition by adult human aortic smooth muscle cells within three-dimensional cocultures." dans: Tissue engineering. Part A, Vol. 21, Issue 7-8, pp. 1455-70, (2015) (PubMed).

Liu, Zou, Zhou, Gong, Wang, Cai, Tan, Fang: "miR-155 Regulates Glioma Cells Invasion and Chemosensitivity by p38 Isforms In Vitro." dans: Journal of cellular biochemistry, Vol. 116, Issue 7, pp. 1213-21, (2015) (PubMed).

Gishto, Farrell, Kothapalli: "Tuning composition and architecture of biomimetic scaffolds for enhanced matrix synthesis by murine cardiomyocytes." dans: Journal of biomedical materials research. Part A, Vol. 103, Issue 2, pp. 693-708, (2015) (PubMed).

Qin, Liu, Li, Liu, Li, Gao, Shao, Zhen: "MMP-2/9-oriented combinations enhance antitumor efficacy of EGFR/HER2-targeting fusion proteins and gemcitabine." dans: Oncology reports, Vol. 32, Issue 1, pp. 121-30, (2014) (PubMed).

Yuan, Yang, Zhou, Gao, Qiu, Fang, Ding, Xiao et al.: "Knockdown of sphingosine kinase 1 inhibits the migration and invasion of human rheumatoid arthritis fibroblast-like synoviocytes by down-regulating the PI3K/AKT activation and MMP-2/9 production in ..." dans: Molecular biology reports, Vol. 41, Issue 8, pp. 5157-65, (2014) (PubMed).

Mazani, Fard, Baghi, Nemati, Mogadam: "Effect of pomegranate juice supplementation on matrix metalloproteinases 2 and 9 following exhaustive exercise in young healthy males." dans: JPMA. The Journal of the Pakistan Medical Association, Vol. 64, Issue 7, pp. 785-90, (2014) (PubMed).

Hopps, Lo Presti, Montana, Canino, Averna, Caimi: "Study of the correlations among some parameters of the oxidative status, gelatinases, and their inhibitors in a group of subjects with metabolic syndrome." dans: Mediators of inflammation, Vol. 2014, pp. 510619, (2014) (PubMed).

Xu, Ling, Zhu, Fan, Zhang: "The effect of 2,3,4',5-tetrahydroxystilbene-2-0-?-D glucoside on neointima formation in a rat artery balloon injury model and its possible mechanisms." dans: European journal of pharmacology, Vol. 698, Issue 1-3, pp. 370-8, (2013) (PubMed).

Tang, Tang, Fang, Liang, Zhang: "Effects of ginsenoside Rh2 on growth and migration of pancreatic cancer cells." dans: World journal of gastroenterology, Vol. 19, Issue 10, pp. 1582-92, (2013) (PubMed).

Hopps, Lo Presti, Montana, Noto, Averna, Caimi: "Gelatinases and their tissue inhibitors in a group of subjects with metabolic syndrome." dans: Journal of investigative medicine : the official publication of the American Federation for Clinical Research, Vol. 61, Issue 6, pp. 978-83, (2013) (PubMed).

Jiang, Huang, Yu, Wu, Zhou, Li: "Overexpression of HTRA1 leads to down-regulation of fibronectin and functional changes in RF/6A cells and HUVECs." dans: PLoS ONE, Vol. 7, Issue 10, pp. e46115, (2013) (PubMed).

Kim, Lee, Choi, Yoo, Yang: "Implication of MMP-9 and urokinase plasminogen activator (uPA) in the activation of pro-matrix metalloproteinase (MMP)-13." dans: Rheumatology international, Vol. 32, Issue 10, pp. 3069-75, (2012) (PubMed).

Yu, Zhu, Mi, Chen, Pan, Wei: "Anti-angiogenic genistein inhibits VEGF-induced endothelial cell activation by decreasing PTK activity and MAPK activation." dans: Medical oncology (Northwood, London, England), Vol. 29, Issue 1, pp. 349-57, (2012) (PubMed).

Pan, Ren, Ma, Liu, Yu, Ji, Pan, Li, Yang, Lv, Shen, Chen, Zhang, Willard, He, Zheng: "High-density lipoprotein of patients with type 2 diabetes mellitus elevates the capability of promoting migration and invasion of breast cancer cells." dans: International journal of cancer. Journal international du cancer, Vol. 131, Issue 1, pp. 70-82, (2012) (PubMed).

Détail du antigène

Antigène: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Autre désignation: MMP2 (MMP2 Produits)

Synonymes: 2-MMP Kit ELISA, CG1794 Kit ELISA, DM2-MMP Kit ELISA, Dm2-MMP Kit ELISA, Dmel\\CG1794 Kit ELISA, MMP2 Kit ELISA, anon-WO0118547.84 Kit ELISA, dm-2MMP Kit ELISA, dmmp2 Kit ELISA, l(2)02353 Kit ELISA, mmp2 Kit ELISA, wu:fa99h12 Kit ELISA, wu:fk89d01 Kit ELISA, fgmmp-2 Kit ELISA, Mmp-2 Kit ELISA, LOC100135793 Kit ELISA, Clg4a Kit ELISA, GelA Kit ELISA, MMP-2 Kit ELISA, CLG4 Kit ELISA, CLG4A Kit ELISA, MMP-II Kit ELISA, MONA Kit ELISA, TBE-1 Kit ELISA, matrix metallopeptidase 2 Kit ELISA, Matrix metalloproteinase 2 Kit ELISA, matrix metalloproteinase 2 Kit ELISA, matrix metalloproteinase-2 Kit ELISA, MMP2 Kit ELISA, Mmp2 Kit ELISA, mmp2 Kit ELISA, LOC657982 Kit ELISA, LOC100135793 Kit ELISA

Sujet:

Protein Function: Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues in association with MMP14.

Background: Type IV collagenase, 72-kD, is officially designated matrix metalloproteinase-2(MMP2). It is also known as gelatinase, 72-kD. MMP-2 plays an essential role in angiogenesis and arteriogenesis, two processes critical to restoration of tissue perfusion after ischemia. MMP-2 expression is increased in tissue ischemia, but the responsible mechanisms remain unknown. Matrix metalloproteinases(MMPs) catalyze extracellular matrix degradation. Control of their activity is a promising target for therapy of diseases characterized by abnormal connective tissue turnover. MMPs are expressed as latent proenzymes that are activated by proteolytic cleavage that triggers a conformational change in the propeptide(cysteine switch). The structure of proMMP-2 reveals how the propeptide shields the catalytic cleft and that the cysteine switch may operate through cleavage of loops essential for propeptide stability. The gene is localized to 16q21 using somatic cell hybrids and in situ hybridization. The standard product used in this kit is recombinant human MMP-2, consisting of 631 amino acids with the molecular mass of 71KDa. The detected MMP-2 includes zymogen and active enzyme.

Synonyms: 72 kDa type IV collagenase,3.4.24.24,72 kDa gelatinase,Gelatinase A,Matrix metalloproteinase-2,MMP-2,TBE-1,PEX,MMP2,CLG4A,

Full Gene Name: 72 kDa type IV collagenase

Cellular Localisation: Isoform 1: Secreted, extracellular space, extracellular matrix. Membrane. Nucleus. Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes.

ID gène: 4313

UniProt: P08253

Pathways: Activation of Innate immune Response