MMP8 Kit ELISA

N° du produit ABIN411332

Détail du produit MMP8 Kit ELISA

Antigène: MMP8 (Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8))

Épitope: AA 21-467

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156-10000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human MMP-8

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Saliva

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: F21-G467

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: F21-G467

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Belongs to the peptidase M10A family.

Tissue Specificity: Neutrophils.

Plaque: Pre-coated

Protocole: human MMP-8 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MMP-8 has been precoated onto 96-well plates. Standards(NSO, F21-G467) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MMP-8 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human MMP-8 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human MMP-8 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma (heparin) or saliva to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human MMP-8 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 1275, Standard deviation: 56.1, CV(%): 4.4
• Sample 2: n=16, Mean(pg/ml): 3563, Standard deviation: 185.3, CV(%): 5.2
• Sample 3: n=16, Mean(pg/ml): 6822, Standard deviation: 395.7, CV(%): 5.8,
• Sample 1: n=24, Mean(pg/ml): 1370, Standard deviation: 69.87, CV(%): 5.1
• Sample 2: n=24, Mean(pg/ml): 3728, Standard deviation: 242.32, CV(%): 6.5
• Sample 3: n=24, Mean(pg/ml): 7142, Standard deviation: 521.4, CV(%): 7.3

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour MMP8 Kit ELISA (ABIN411332)

Kumar, Jain, Chauhan, Bharate, Koli, Kumar: "Influence of different forms and materials (zirconia or titanium) of abutments in periimplant soft-tissue healing using matrix metalloproteinase-8: A randomized pilot study." dans: The Journal of prosthetic dentistry, (2017) (PubMed).

Stio, Martinesi, Treves, Borgioli: "In vitro response of human peripheral blood mononuclear cells to AISI 316L austenitic stainless steel subjected to nitriding and collagen coating treatments." dans: Journal of materials science. Materials in medicine, Vol. 26, Issue 2, pp. 100, (2015) (PubMed).

Akbari, Prabhuji, Karthikeyan, Raghunatha, Narayanan: "Analysis of matrix metalloproteinase-8 levels in gingival crevicular fluid and whole mouth fluid among smokers and nonsmokers using enzyme-linked immune-sorbent assay and a novel chair-side test." dans: Journal of Indian Society of Periodontology, Vol. 19, Issue 5, pp. 525-30, (2015) (PubMed).

Détail du antigène

Antigène: MMP8 (Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8))

Autre désignation: MMP8 (MMP8 Produits)

Synonymes: MMP8 Kit ELISA, BB138268 Kit ELISA, CLG1 Kit ELISA, HNC Kit ELISA, MMP-8 Kit ELISA, PMNL-CL Kit ELISA, matrix metallopeptidase 8 Kit ELISA, neutrophil collagenase Kit ELISA, MMP8 Kit ELISA, Mmp8 Kit ELISA, LOC100339790 Kit ELISA

Sujet:

Protein Function: Can degrade fibrillar type I, II, and III collagens.

Background: Matrix metalloproteinase 8(MMP8) also called neutrophil collagenase. Neutrophil collagenase, a member of the family of matrix metalloproteinases, is distinct from the collagenase of skin fibroblasts and synovial cells in substrate specificity and immunologic crossreactivity. MMP8, an enzyme that degrades fibrillar collagens imparting strength to the fetal membranes, is expressed by leukocytes and chorionic cytotrophoblast cells. The human neutrophil collagenase(HNC) cDNA clone has been sequenced and shown to encode a 467-residue protein. Neutrophil collagenase has been found to possess 57 % identity with the deduced protein sequence for fibroblast collagenase with 72 % chemical similarity. Certain regions of the molecule, including the putative zinc-binding region, are highly conserved. When compared with the published sequence for fibroblast collagenase, neutrophil collagenase contains four additional sites for glycosylation. The standard product used in this kit is natural, isolating from human MMP-8. The detected MMP-8 includes zymogen and active enzyme.

Synonyms: Neutrophil collagenase,3.4.24.34,Matrix metalloproteinase-8,MMP-8,PMNL collagenase,PMNL-CL,MMP8,CLG1,

Full Gene Name: Neutrophil collagenase

Cellular Localisation: Cytoplasmic granule. Secreted, extracellular space, extracellular matrix . Stored in intracellular granules.

ID gène: 4317

UniProt: P22894