Neurotrophin 3 Kit ELISA

N° du produit ABIN411338

Détail du produit Neurotrophin 3 Kit ELISA

Antigène: Neurotrophin 3 (NTF3)

Épitope: AA 139-257

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6-1000 pg/mL

Seuil minimal de détection: 15.6 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Neurotrophin-3

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: Expression system for standard: sf21
Immunogen sequence: Y139-T257

Réactivité croisée (Details): There is cross-reactivity with NT-4<2.5 % , and no detectable cross-reactivity with any other cytokine.

Homologie: Bovine,Chicken,Horse,Monkey,Rabbit

Sensibilité: <2pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: sf21
Immunogen sequence: Y139-T257

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Tissue Specificity: Brain and peripheral tissues.

Plaque: Pre-coated

Protocole: human Neurotrophin-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Neurotrophin-3 has been precoated onto 96-well plates. Standards(sf21, Y139-T257) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Neurotrophin-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Neurotrophin-3 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human Neurotrophin-3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernatants or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Neurotrophin-3 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 93, Standard deviation: 4, CV(%): 4.3
• Sample 2: n=16, Mean(pg/ml): 357, Standard deviation: 17.5, CV(%): 4.9
• Sample 3: n=16, Mean(pg/ml): 657, Standard deviation: 34.2, CV(%): 5.2,
• Sample 1: n=24, Mean(pg/ml): 146, Standard deviation: 7.88, CV(%): 5.4
• Sample 2: n=24, Mean(pg/ml): 375, Standard deviation: 22.9, CV(%): 6.1
• Sample 3: n=24, Mean(pg/ml): 732, Standard deviation: 47.58, CV(%): 6.5

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour Neurotrophin 3 Kit ELISA (ABIN411338)

Wang, Li, Kong, Zhao, Yang, Chen, Yan: "Enhanced expression of neurotrophic factors in the injured spinal cord through vaccination with myelin basic protein-derived peptide pulsed dendritic cells." dans: Spine, Vol. 40, Issue 2, pp. 95-101, (2015) (PubMed).

Guo, Sun, Xu, Zhao, Peng, Wang: "Human umbilical cord mesenchymal stem cells promote peripheral nerve repair via paracrine mechanisms." dans: Neural regeneration research, Vol. 10, Issue 4, pp. 651-8, (2015) (PubMed).

Zhao, Wang, Peng, Ren, Zhang, Guo, Xu, Lu: "Improvement in nerve regeneration through a decellularized nerve graft by supplementation with bone marrow stromal cells in fibrin." dans: Cell transplantation, Vol. 23, Issue 1, pp. 97-110, (2014) (PubMed).

Forsyth, Krishna, Lawn, Valadez, Qu, Fenstermacher, Fournier, Potthast, Chinnaiyan, Gibney, Zeinieh, Barker, Carter, Cooper, Kenchappa: "p75 neurotrophin receptor cleavage by ?- and ?-secretases is required for neurotrophin-mediated proliferation of brain tumor-initiating cells." dans: The Journal of biological chemistry, Vol. 289, Issue 12, pp. 8067-85, (2014) (PubMed).

Détail du antigène

Antigène: Neurotrophin 3 (NTF3)

Autre désignation: NTF3 (NTF3 Produits)

Synonymes: HDNF Kit ELISA, NGF-2 Kit ELISA, NGF2 Kit ELISA, NT3 Kit ELISA, NT-3 Kit ELISA, wu:fl98d04 Kit ELISA, hdnf Kit ELISA, ngf-2 Kit ELISA, ngf2 Kit ELISA, nt-3 Kit ELISA, nt3 Kit ELISA, NTF3 Kit ELISA, AI316846 Kit ELISA, AI835689 Kit ELISA, Nt3 Kit ELISA, Ntf-3 Kit ELISA, neurotrophin 3 Kit ELISA, neurotrophin 3 S homeolog Kit ELISA, neurotrophin 3 L homeolog Kit ELISA, NTF3 Kit ELISA, ntf3 Kit ELISA, ntf3.S Kit ELISA, Ntf3 Kit ELISA, ntf3.L Kit ELISA

Sujet:

Protein Function: Seems to promote the survival of visceral and proprioceptive sensory neurons.

Background: Neurotrophin-3(NT-3) is a new member of the nerve growth factor gene family, which plays an important role in the development and maintenance of the vertebrate nervous system. NT-3 and its receptor, called neurotrophic tyrosine kinase receptor type 3(Ntrk3, also called TrkC), are expressed early and throughout embryogenesis. NT-3 is one of five neurotrophin growth factors which shape the development of the nervous system by regulating neuronal survival and differentiation. NT-3 may be one of the central nervous system-derived factors that mediate neural crest(NC) cell proliferation in vivo. NT-3 has been mapped to human chromosome 12p and mouse chromosome 6.1

Synonyms: Neurotrophin-3,NT-3,HDNF,Nerve growth factor 2,NGF-2,Neurotrophic factor,NTF3,

Full Gene Name: Neurotrophin-3

Cellular Localisation: Secreted.

ID gène: 4908

UniProt: P20783

Pathways: Signalisation RTK, Neurotrophin Signaling Pathway