Selectin E/CD62e Kit ELISA

N° du produit ABIN411350

Détail du produit Selectin E/CD62e Kit ELISA

Antigène: Selectin E/CD62e (SELE) (Selectin E (SELE))

Épitope: AA 22-556

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 125-8000 pg/mL

Seuil minimal de détection: 125 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human sE-Selectin

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate)

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: W22-P556

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <4pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: W22-P556

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Belongs to the selectin/LECAM family.

Plaque: Pre-coated

Protocole: human sE-Selectin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for sE-Selectin has been precoated onto 96-well plates. Standards(NSO, W22-P556) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for sE-Selectin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human sE-Selectin amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 8000pg/mL, 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL human sE-Selectin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human sE-Selectin standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(ng/ml): 1.7, Standard deviation: 0.10, CV(%): 5.9
• Sample 2: n=16, Mean(ng/ml): 3.1, Standard deviation: 0.16, CV(%): 5.2
• Sample 3: n=16, Mean(ng/ml): 4.5, Standard deviation: 0.31, CV(%): 6.9,
• Sample 1: n=24, Mean(ng/ml): 1.6, Standard deviation: 0.13, CV(%): 8.1
• Sample 2: n=24, Mean(ng/ml): 3.2, Standard deviation: 0.21, CV(%): 6.6
• Sample 3: n=24, Mean(ng/ml): 4.5, Standard deviation: 0.33, CV(%): 7.3

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour Selectin E/CD62e Kit ELISA (ABIN411350)

Dehghan, Sabri, Javanmard, Ahmadi, Mansourian: "Neurally mediated syncope: Is it really an endothelial dysfunction?" dans: Anatolian journal of cardiology, Vol. 16, Issue 9, pp. 701-6, (2018) (PubMed).

Ahluwalia, Misto, Vozzi, Magliaro, Mattei, Marescotti, Avogaro, Iori: "Systemic and vascular inflammation in an in-vitro model of central obesity." dans: PLoS ONE, Vol. 13, Issue 2, pp. e0192824, (2018) (PubMed).

Park, Willoughby, Song, Leutholtz, Koh: "Exercise-induced changes in stress hormones and cell adhesion molecules in obese men." dans: Journal of inflammation research, Vol. 11, pp. 69-75, (2018) (PubMed).

Majidinia, Rasmi, Khadem Ansari, Seyed-Mohammadzad, Saboory, Shirpoor: "Metoprolol Improves Endothelial Function in Patients with Cardiac Syndrome X." dans: Iranian journal of pharmaceutical research : IJPR, Vol. 15, Issue 3, pp. 561-566, (2016) (PubMed).

Bilgic, Yilmaz, Bozkurt, Celik, Bilgic, Gurel, Kirbas, Bavbek, Akcay: "Relationship of late arteriovenous fistula stenosis with soluble E-selectin and soluble EPCR in chronic hemodialysis patients with arteriovenous fistula." dans: Clinical and experimental nephrology, Vol. 19, Issue 1, pp. 133-9, (2015) (PubMed).

Sabri, Dehghan, Yaghini, Nasiri, Mansourian, Khalifehsoltani: "Endothelial dysfunction state in migraine headache and neutrally mediated syncope in children and young adults." dans: Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences, Vol. 20, Issue 8, pp. 771-6, (2015) (PubMed).

Asgary, Keshvari, Sahebkar, Hashemi, Rafieian-Kopaei: "Clinical investigation of the acute effects of pomegranate juice on blood pressure and endothelial function in hypertensive individuals." dans: ARYA atherosclerosis, Vol. 9, Issue 6, pp. 326-31, (2014) (PubMed).

Asgary, Keshvari, Afshani, Amiri, Laher, Javanmard: "Effect of fresh orange juice intake on physiological characteristics in healthy volunteers." dans: ISRN nutrition, Vol. 2014, pp. 405867, (2014) (PubMed).

Détail du antigène

Antigène: Selectin E/CD62e (SELE) (Selectin E (SELE))

Autre désignation: SELE (SELE Produits)

Synonymes: SELE Kit ELISA, CD62E Kit ELISA, ELAM Kit ELISA, ELAM1 Kit ELISA, ESEL Kit ELISA, LECAM2 Kit ELISA, E-selectin Kit ELISA, Elam Kit ELISA, selectin E Kit ELISA, selectin, endothelial cell Kit ELISA, SELE Kit ELISA, Sele Kit ELISA

Sujet:

Protein Function: Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis. .

Background: E-selectin, also called endothelial-leukocyte adhesion molecule-1(ELAM-1), is a cell surface glycoprotein expressed by cytokine-activated endothelium that mediates the adhesion of blood neutrophils. An increased expression of E-selectin has been observed in the arterial endothelium interacting with lymphocytes and macrophages in human atherosclerotic lesions. E-selectin plays a critical role in mediating tissue-specific homing of T cells into skin, and of primitive hematopoietic progenitor cells(HPCs) into bone marrow(BM). A structurally and functionally related group of genes, lymph node homing receptor(LHR), granule membrane protein 140(GMP-140), and endothelial leukocyte adhesion molecule 1(ELAM-1) are shown to constitute a gene cluster on mouse and human chromosome 1. The standard product used in this kit is recombinant human E-Selectin, excluding intercellular E-Selectin and transmembrane domain. It consists of totally 535 amino acids with the molecular mass of 58.6KDa.

Synonyms: E-selectin,CD62 antigen-like family member E,Endothelial leukocyte adhesion molecule 1,ELAM-1,Leukocyte-endothelial cell adhesion molecule 2,LECAM2,CD62E,SELE,ELAM1,

Full Gene Name: E-selectin

Cellular Localisation: Cell membrane, Single-pass type I membrane protein.

ID gène: 6401

UniProt: P16581

Pathways: Thromboxane A2 Receptor Signaling