TGFB1 Kit ELISA

N° du produit ABIN411353

Détail du produit TGFB1 Kit ELISA

Antigène: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Épitope: AA 279-390

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6-1000 pg/mL

Seuil minimal de détection: 15.6 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of activated Human TGF beta 1

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (EDTA), Urine

Analytical Method: Quantitative

Specificité: Expression system for standard: CHO
Immunogen sequence: A279-S390

Réactivité croisée (Details): There is cross-reactivity with TGF beta 2, TGF beta 3, TGF beta 5<1 % .

Homologie: Bovine,Canine,Monkey,Rabbit

Sensibilité: <1pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: CHO
Immunogen sequence: A279-S390

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Belongs to the TGF-beta family.

Tissue Specificity: Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Colocalizes with ASPN in chondrocytes within OA lesions of articular cartilage. .

Plaque: Pre-coated

Protocole: human TGF beta 1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TGF beta 1 has been precoated onto 96-well plates. Standards(CHO, A279-S390) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TGF beta 1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TGF beta 1 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human TGF beta 1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of activated human in cell culture supernates, serum, plasma (EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human TGF beta 1 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 135, Standard deviation: 5.94, CV(%): 4.4
• Sample 2: n=16, Mean(pg/ml): 264, Standard deviation: 13.73, CV(%): 5.2
• Sample 3: n=16, Mean(pg/ml): 521, Standard deviation: 18.8, CV(%): 3.6,
• Sample 1: n=24, Mean(pg/ml): 152, Standard deviation: 7.6, CV(%): 5
• Sample 2: n=24, Mean(pg/ml): 270, Standard deviation: 17.3, CV(%): 6.4
• Sample 3: n=24, Mean(pg/ml): 543, Standard deviation: 32.04, CV(%): 5.9

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour TGFB1 Kit ELISA (ABIN411353)

Li, Wang, Wang, Sun, Cai, Liu, Zhang, Lu, Li, Chen, Chen, Jiang: "Combination epidermal growth factor receptor variant III peptide-pulsed dendritic cell vaccine with miR-326 results in enhanced killing on EGFRvIII-positive cells." dans: Oncotarget, Vol. 8, Issue 16, pp. 26256-26268, (2018) (PubMed).

Liu, Zhen, Song, Chen, Sun, Li, Zhou, Yan, Ding, Sun: "Low-dose lymphocyte immunotherapy rebalances the peripheral blood Th1/Th2/Treg paradigm in patients with unexplained recurrent miscarriage." dans: Reproductive biology and endocrinology : RB&E, Vol. 15, Issue 1, pp. 95, (2018) (PubMed).

Zhu, Gu, Ma, Gao, Liu, Xiao, Wang, Chen, Li: "Biomarkers for Pulmonary Inflammation and Fibrosis and Lung Ventilation Function in Chinese Occupational Refractory Ceramic Fibers-Exposed Workers." dans: International journal of environmental research and public health, Vol. 15, Issue 1, (2018) (PubMed).

Jiang, Tan, Cai, Zhao, Mao, Zhang, Xu, Yan, Qian, Yan: "Human Umbilical Cord MSC-Derived Exosomes Suppress the Development of CCl4-Induced Liver Injury through Antioxidant Effect." dans: Stem cells international, Vol. 2018, pp. 6079642, (2018) (PubMed).

Gheybi, Farrokhi, Ravanbod, Ostovar, Mehrzad, Nematollahi: "The correlation of CD19 + CD24 + CD38 + B cells and other clinicopathological variables with the proportion of circulating Tregs in breast cancer patients." dans: Breast cancer (Tokyo, Japan), (2017) (PubMed).

Wang, Wang, Wang, Hong, Han, Chen, Chen: "HMGB1 induces lung fibroblast to myofibroblast differentiation through NF‑κB‑mediated TGF‑β1 release." dans: Molecular medicine reports, Vol. 15, Issue 5, pp. 3062-3068, (2017) (PubMed).

Xuanfei, Hao, Zhujun, Yanming, Jianping: "Imidazoline I2 receptor inhibitor idazoxan regulates the progression of hepatic fibrosis via Akt-Nrf2-Smad2/3 signaling pathway." dans: Oncotarget, Vol. 8, Issue 13, pp. 21015-21030, (2017) (PubMed).

Bu, Yu, Wu, Zhong, Yang, Chen: "Normalization of regulatory T cells, serum TGF-β1, and LTN after 5-aminolevulinic acid-photodynamic therapy in patients with condyloma acuminate." dans: Experimental and therapeutic medicine, Vol. 13, Issue 6, pp. 3327-3332, (2017) (PubMed).

Cheng, Ge, Lan, Ma, Chi, Kuang, Sun, Zhao, Liu, Feng, Huang, Luo, Li, Zhang, Hu, Xu, Liu, Huo, Deng, Yang, Xi, Zhang, Siegenthaler, Chen: "Zoledronate dysregulates fatty acid metabolism in renal tubular epithelial cells to induce nephrotoxicity." dans: Archives of toxicology, Vol. 92, Issue 1, pp. 469-485, (2017) (PubMed).

Wu, Yu, Cai, Lu, Jiang, Liu, Li, Feng, Gao, Zhu, Yu, Yuan, Cui: "Effects of increased Kindlin-2 expression in bladder cancer stromal fibroblasts." dans: Oncotarget, Vol. 8, Issue 31, pp. 50692-50703, (2017) (PubMed).

Santagata, Napolitano, DAlterio, Desicato, Maro, Marinelli, Fragale, Buoncervello, Persico, Gabriele, Novellino, Longo, Pignata, Perdonà, Scala: "Targeting CXCR4 reverts the suppressive activity of T-regulatory cells in renal cancer." dans: Oncotarget, Vol. 8, Issue 44, pp. 77110-77120, (2017) (PubMed).

Metzler, Pritz, Riml, Romani, Tuertscher, Steinbichler, Dejaco, Riechelmann, Dudás: "Separation of cell survival, growth, migration, and mesenchymal transdifferentiation effects of fibroblast secretome on tumor cells of head and neck squamous cell carcinoma." dans: Tumour biology, Vol. 39, Issue 11, pp. 1010428317705510, (2017) (PubMed).

Zhuang, Lu, Shen, Huang, Shen, Zheng, Huang, Yan, Guo: "TGFβ1 secreted by cancer-associated fibroblasts induces epithelial-mesenchymal transition of bladder cancer cells through lncRNA-ZEB2NAT." dans: Scientific reports, Vol. 5, pp. 11924, (2016) (PubMed).

Yılmazlar, Karabayırlı, Gözdemir, Usta, Peker, Namuslu, Erdamar: "The effect of positive end-expiratory pressure on inflammatory cytokines during laparoscopic cholecystectomy." dans: Saudi medical journal, Vol. 36, Issue 11, pp. 1358-62, (2016) (PubMed).

Jiang, Chen, Wang, Lin, Hu, Fang: "Changes in interleukin-17 and transforming growth factor beta 1 levels in serum and bronchoalveolar lavage fluid and their clinical significance among children with asthma." dans: Translational pediatrics, Vol. 2, Issue 4, pp. 154-9, (2016) (PubMed).

Zhang, Chen, Chen, Meng, Xiong, Liu, Jin, Tao, Wu, Sun: "TGF-β/BAMBI pathway dysfunction contributes to peripheral Th17/Treg imbalance in chronic obstructive pulmonary disease." dans: Scientific reports, Vol. 6, pp. 31911, (2016) (PubMed).

Hu, Xu, Yao, Hong, Wu, Pei, Chang, Ding, Gao, Ye, Wang, Hei, Zhou: "MiR-663 inhibits radiation-induced bystander effects by targeting TGFB1 in a feedback mode." dans: RNA biology, Vol. 11, Issue 9, pp. 1189-98, (2015) (PubMed).

Li, Pan, Qiu: "Imbalances of Th17 and Treg cells and their respective cytokines in COPD patients by disease stage." dans: International journal of clinical and experimental medicine, Vol. 7, Issue 12, pp. 5324-9, (2015) (PubMed).

Wang, Yao, Xiong, Wu, Tang, Li: "Evaluation of the inflammatory response in macrophages stimulated with exosomes secreted by Mycobacterium avium-infected macrophages." dans: BioMed research international, Vol. 2015, pp. 658421, (2015) (PubMed).

Zhang, Jiang, Chen, Wang, Feng, Tao, Qin: "Serum Levels of IL-1 ? , IL-6, TGF- ? , and MMP-9 in Patients Undergoing Carotid Artery Stenting and Regulation of MMP-9 in a New In Vitro Model of THP-1 Cells Activated by Stenting." dans: Mediators of inflammation, Vol. 2015, pp. 956082, (2015) (PubMed).

Détail du antigène

Antigène: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Autre désignation: TGFB1 (TGFB1 Produits)

Synonymes: CED Kit ELISA, DPD1 Kit ELISA, LAP Kit ELISA, TGFB Kit ELISA, TGFbeta Kit ELISA, TGF-beta Kit ELISA, TGF-BETA-1 Kit ELISA, TGF-beta5 Kit ELISA, ced Kit ELISA, dpd1 Kit ELISA, lap Kit ELISA, tgf-beta Kit ELISA, tgfb Kit ELISA, tgfb5 Kit ELISA, tgfbeta Kit ELISA, TGF-beta1 Kit ELISA, TGFbeta1 Kit ELISA, Tgfb Kit ELISA, Tgfb-1 Kit ELISA, ai39657 Kit ELISA, tgfb1 Kit ELISA, wu:fb13a07 Kit ELISA, xx:ai39657 Kit ELISA, TGFB1 Kit ELISA, csd Kit ELISA, cdb1 Kit ELISA, cdg2 Kit ELISA, csd1 Kit ELISA, csd2 Kit ELISA, csd3 Kit ELISA, ebmd Kit ELISA, lcd1 Kit ELISA, bigh3 Kit ELISA, cdgg1 Kit ELISA, betaig-h3 Kit ELISA, TGFB4 Kit ELISA, transforming growth factor beta 1 Kit ELISA, transforming growth factor beta-1 Kit ELISA, transforming growth factor beta 1 L homeolog Kit ELISA, transforming growth factor, beta 1 Kit ELISA, transforming growth factor, beta 1a Kit ELISA, transforming growth factor beta induced L homeolog Kit ELISA, TGFB1 Kit ELISA, Tgfb1 Kit ELISA, tgfb1.L Kit ELISA, tgfb1a Kit ELISA, tgfbi.L Kit ELISA

Sujet:

Protein Function: Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Can promote either T- helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner. At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regulation of IL-17 expression, favoring Treg cell development. At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells. .

Background: Transforming growth factor-beta1(TGF-beta1) is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. Many cells synthesize TGF-beta and essentially all of them have specific receptors for this peptide. TGF-beta regulates the actions of many other peptide growth factors and determines a positive or negative direction of their effects.TGFbeta1 is known for its potent and diverse biological effects, including immune regulation, and cell growth and differentiation.TGFbeta1 is also an important mediator of bone remodeling.TGFbeta1, a potent keratinocyte growth inhibitor, has been shown to be overexpressed in keratinocytes in certain inflammatory skin diseases and has been thought to counteract the effects of other growth factors at the site of inflammation.TGF-beta1, a multifunctional cytokine with fibrogenic properties, has been implicated in the pathogenesis of the vascular and target organ complications of hypertension. TGF-beta1 may also regulate blood pressure via stimulation of endothelin-1 and/or renin secretion. TGFbeta1 is secreted as a latent form, which consists of its mature form and a latency-associated peptide(beta1-LAP) in either the presence or the absence of additional latent TGF-beta1-binding protein. The standard product used in this kit is recombinant TGFbeta1 with the molecular mass of 25KDa.

Synonyms: Transforming growth factor beta-1,TGF-beta-1,Latency-associated peptide,LAP,TGFB1,TGFB,

Full Gene Name: Transforming growth factor beta-1

Cellular Localisation: Secreted, extracellular space, extracellular matrix.

ID gène: 7040

UniProt: P01137

Pathways: EGFR Signaling Pathway, Dopaminergic Neurogenesis, Cellular Response to Molecule of Bacterial Origin, Glycosaminoglycan Metabolic Process, Regulation of Leukocyte Mediated Immunity, Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Ribonucleoside Biosynthetic Process, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy, Cancer Immune Checkpoints