TIMP1 Kit ELISA

N° du produit ABIN411358

Détail du produit TIMP1 Kit ELISA

Antigène: TIMP1 (TIMP Metallopeptidase Inhibitor 1 (TIMP1))

Épitope: AA 24-207

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156-10000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TIMP-1

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Saliva

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: C24-A207

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <5pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: C24-A207

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Belongs to the protease inhibitor I35 (TIMP) family.

Tissue Specificity: Detected in rheumatoid synovial fluid (at protein level). .

Plaque: Pre-coated

Protocole: human TIMP-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TIMP-1 has been precoated onto 96-well plates. Standards(NSO, C24-A207) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TIMP-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TIMP-1 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human TIMP-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or saliva to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human TIMP-1 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(ng/ml): 1.21, Standard deviation: 0.057, CV(%): 4.7
• Sample 2: n=16, Mean(ng/ml): 3.28, Standard deviation: 0.171, CV(%): 5.2
• Sample 3: n=16, Mean(ng/ml): 7.06, Standard deviation: 0.424, CV(%): 6,
• Sample 1: n=24, Mean(ng/ml): 1.34, Standard deviation: 0.071, CV(%): 5.3
• Sample 2: n=24, Mean(ng/ml): 3.54, Standard deviation: 0.248, CV(%): 7
• Sample 3: n=24, Mean(ng/ml): 6.32, Standard deviation: 0.43, CV(%): 6.8

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour TIMP1 Kit ELISA (ABIN411358)

Sollazzo, Forte, Polverelli, Romano, Perricone, Rossi, Ottaviani, Luatti, Martinelli, Vianelli, Cavo, Palandri, Catani: "Crucial factors of the inflammatory microenvironment (IL-1β/TNF-α/TIMP-1) promote the maintenance of the malignant hemopoietic clone of myelofibrosis: an in vitro study." dans: Oncotarget, Vol. 7, Issue 28, pp. 43974-43988, (2018) (PubMed).

Forte, Salvestrini, Corradi, Rossi, Catani, Lemoli, Cavo, Curti: "The tissue inhibitor of metalloproteinases-1 (TIMP-1) promotes survival and migration of acute myeloid leukemia cells through CD63/PI3K/Akt/p21 signaling." dans: Oncotarget, Vol. 8, Issue 2, pp. 2261-2274, (2018) (PubMed).

Liu, Zhong, He, Wei, Zheng, Zhang, Bai, Zhong, Zhong: "Effect of low-dose, long-term roxithromycin on airway inflammation and remodeling of stable noncystic fibrosis bronchiectasis." dans: Mediators of inflammation, Vol. 2014, pp. 708608, (2015) (PubMed).

Deng, Ling, Liu, Zhao, Lv: "Decreased expression of matrix metalloproteinase-1 in the maternal umbilical serum, trophoblasts and decidua leads to preeclampsia." dans: Experimental and therapeutic medicine, Vol. 9, Issue 3, pp. 992-998, (2015) (PubMed).

Gishto, Farrell, Kothapalli: "Tuning composition and architecture of biomimetic scaffolds for enhanced matrix synthesis by murine cardiomyocytes." dans: Journal of biomedical materials research. Part A, Vol. 103, Issue 2, pp. 693-708, (2015) (PubMed).

Stio, Martinesi, Treves, Borgioli: "In vitro response of human peripheral blood mononuclear cells to AISI 316L austenitic stainless steel subjected to nitriding and collagen coating treatments." dans: Journal of materials science. Materials in medicine, Vol. 26, Issue 2, pp. 100, (2015) (PubMed).

Simmers, Gishto, Vyavahare, Kothapalli: "Nitric oxide stimulates matrix synthesis and deposition by adult human aortic smooth muscle cells within three-dimensional cocultures." dans: Tissue engineering. Part A, Vol. 21, Issue 7-8, pp. 1455-70, (2015) (PubMed).

Cavdar, Ozbal, Celik, Ergur, Guneli, Ural, Camsari, Guner: "The effects of alpha-lipoic acid on MMP-2 and MMP-9 activities in a rat renal ischemia and re-perfusion model." dans: Biotechnic & histochemistry : official publication of the Biological Stain Commission, Vol. 89, Issue 4, pp. 304-14, (2014) (PubMed).

Hopps, Lo Presti, Montana, Canino, Averna, Caimi: "Study of the correlations among some parameters of the oxidative status, gelatinases, and their inhibitors in a group of subjects with metabolic syndrome." dans: Mediators of inflammation, Vol. 2014, pp. 510619, (2014) (PubMed).

Li, Zhao, Ren, Xie, Ma, Rong: "Increased expression of matrix metalloproteinase-9 associated with gastric ulcer recurrence." dans: World journal of gastroenterology, Vol. 19, Issue 28, pp. 4590-5, (2014) (PubMed).

Xu, Ling, Zhu, Fan, Zhang: "The effect of 2,3,4',5-tetrahydroxystilbene-2-0-?-D glucoside on neointima formation in a rat artery balloon injury model and its possible mechanisms." dans: European journal of pharmacology, Vol. 698, Issue 1-3, pp. 370-8, (2013) (PubMed).

Hopps, Lo Presti, Montana, Noto, Averna, Caimi: "Gelatinases and their tissue inhibitors in a group of subjects with metabolic syndrome." dans: Journal of investigative medicine : the official publication of the American Federation for Clinical Research, Vol. 61, Issue 6, pp. 978-83, (2013) (PubMed).

Kim, Lee, Choi, Yoo, Yang: "Implication of MMP-9 and urokinase plasminogen activator (uPA) in the activation of pro-matrix metalloproteinase (MMP)-13." dans: Rheumatology international, Vol. 32, Issue 10, pp. 3069-75, (2012) (PubMed).

Détail du antigène

Antigène: TIMP1 (TIMP Metallopeptidase Inhibitor 1 (TIMP1))

Autre désignation: TIMP1 (TIMP1 Produits)

Synonymes: TIMP-1 Kit ELISA, TIMP1 Kit ELISA, DKFZp468A0912 Kit ELISA, CLGI Kit ELISA, EPA Kit ELISA, EPO Kit ELISA, HCI Kit ELISA, TIMP Kit ELISA, Timp Kit ELISA, Clgi Kit ELISA, TIMP metallopeptidase inhibitor 1 Kit ELISA, tissue inhibitor of metalloproteinase 1 Kit ELISA, TIMP1 Kit ELISA, Timp1 Kit ELISA

Sujet:

Protein Function: Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling. Mediates erythropoiesis in vitro, but, unlike IL3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. .

Background: The tissue inhibitor of metalloproteinases 1(TIMP1) is also called erythroid-potentiating activity(EPA). The X-linked gene for human TIMP1 is expressed in some but not all inactive X-containing somatic-cell hybrids, suggesting that this gene is either prone to reactivation or variable in its inactivation. Purified EPA specifically stimulates human and murine cells of the erythroid lineage, unlike murine interleukin-3(IL-3) which stimulates precursor cells from all haematopoietic lineages. TIMP1 is thought to play a regulatory role in connective tissues by forming inactive complexes with those metalloproteinases that are normally responsible for connective tissue turnover. The human gene encoding TIMP has been mapped to the X chromosome in the region Xp11.1-p11.4. The standard product used in this kit is natural TIMP-1 with the molecular mass of 22KDa.

Synonyms: Metalloproteinase inhibitor 1,Erythroid-potentiating activity,EPA,Fibroblast collagenase inhibitor,Collagenase inhibitor,Tissue inhibitor of metalloproteinases 1,TIMP-1,TIMP1,CLGI, TIMP,

Full Gene Name: Metalloproteinase inhibitor 1

Cellular Localisation: Secreted.

ID gène: 7076

UniProt: P01033