PLAU Kit ELISA

N° du produit ABIN411365

Détail du produit PLAU Kit ELISA

Antigène: PLAU (Plasminogen Activator, Urokinase (PLAU))

Épitope: AA 1-431

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5-4000 pg/mL

Seuil minimal de détection: 62.5 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human uPA/PLAU

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: M1-L431

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <5pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: M1-L431

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Belongs to the peptidase S1 family.

Tissue Specificity: Expressed in the prostate gland and prostate cancers. .

Plaque: Pre-coated

Protocole: human uPA ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for uPA has been precoated onto 96-well plates. Standards(NSO, M1-L431) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for uPA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human uPA amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human uPA standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human uPA standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 126, Standard deviation: 5.92, CV(%): 4.7
• Sample 2: n=16, Mean(pg/ml): 1895, Standard deviation: 64.43, CV(%): 3.4
• Sample 3: n=16, Mean(pg/ml): 2619, Standard deviation: 138.8, CV(%): 5.3,
• Sample 1: n=24, Mean(pg/ml): 250, Standard deviation: 13.5, CV(%): 5.4
• Sample 2: n=24, Mean(pg/ml): 1568, Standard deviation: 95.65, CV(%): 6.1
• Sample 3: n=24, Mean(pg/ml): 2755, Standard deviation: 187.3, CV(%): 6.8

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour PLAU Kit ELISA (ABIN411365)

Bi, Zeng, Zhao, Wei, Yu, Wang, Yu, Cao, Shan, Wei: "miR-181a Induces Macrophage Polarized to M2 Phenotype and Promotes M2 Macrophage-mediated Tumor Cell Metastasis by Targeting KLF6 and C/EBPα." dans: Molecular therapy. Nucleic acids, Vol. 5, Issue 9, pp. e368, (2016) (PubMed).

Bronckaers, Hilkens, Fanton, Struys, Gervois, Politis, Martens, Lambrichts: "Angiogenic properties of human dental pulp stem cells." dans: PLoS ONE, Vol. 8, Issue 8, pp. e71104, (2013) (PubMed).

Kim, Lee, Choi, Yoo, Yang: "Implication of MMP-9 and urokinase plasminogen activator (uPA) in the activation of pro-matrix metalloproteinase (MMP)-13." dans: Rheumatology international, Vol. 32, Issue 10, pp. 3069-75, (2012) (PubMed).

Jiang, Zhang, Zhou, Zhang, Li, Zhang, Zhang, Qiao, Kong, Ma, Chen: "Relationships of uPA and VEGF expression in esophageal cancer and microvascular density with tumorous invasion and metastasis." dans: Asian Pacific journal of cancer prevention : APJCP, Vol. 13, Issue 7, pp. 3379-83, (2012) (PubMed).

Wang, Li, Han, Li, Mi, Sun, Song, Han, Zhao, Wang: "Integrin ?3 and its ligand regulate the expression of uPA through p38 MAPK in breast cancer." dans: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, Vol. 118, Issue 12, pp. 909-17, (2010) (PubMed).

Yang, Shi, Li, Yi: "Effects of shRNA targeting maspin on invasion of gastric carcinoma SGC7901 cell line." dans: Oncology reports, Vol. 25, Issue 1, pp. 259-65, (2010) (PubMed).

Détail du antigène

Antigène: PLAU (Plasminogen Activator, Urokinase (PLAU))

Autre désignation: PLAU (PLAU Produits)

Synonymes: PLAU Kit ELISA, u-PA Kit ELISA, ATF Kit ELISA, BDPLT5 Kit ELISA, QPD Kit ELISA, UPA Kit ELISA, URK Kit ELISA, uPA Kit ELISA, UPAM Kit ELISA, plasminogen activator, urokinase Kit ELISA, urokinase-type plasminogen activator Kit ELISA, PLAU Kit ELISA, CpipJ_CPIJ002131 Kit ELISA, CpipJ_CPIJ006543 Kit ELISA, CpipJ_CPIJ013396 Kit ELISA, CpipJ_CPIJ013623 Kit ELISA, Plau Kit ELISA

Sujet:

Protein Function: Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.

Background: Plasminogen activator,urokinase(PLAU, uPA) converts plasminogen to plasmin. Plasmin is involved in processing of amyloid precursor protein and degrades secreted and aggregated amyloid-beta, a hallmark of Alzheimer disease(AD). Urokinase has a molecular mass of about 54 kD and is composed of 2 disulfide-linked chains, A and B, of molecular masses 18 kD and 33 kD, respectively. It localized on 10q24. uPA facilitates cell migration by localizing proteolisys on the cell surface and by inducing intracellular signalling pathways. In human vascular smooth muscle cell(VSMC), uPA stimulates migration via the uPA receptor(uPAR) signalling complex containing TYK2 and phosphatidylinositol 3-kinase(PI3-K).

Synonyms: Urokinase-type plasminogen activator,U-plasminogen activator,uPA,3.4.21.73,Urokinase-type plasminogen activator long chain A,Urokinase-type plasminogen activator short chain A,Urokinase-type plasminogen activator chain B,PLAU,

Full Gene Name: Urokinase-type plasminogen activator

Cellular Localisation: Secreted.

ID gène: 5328

UniProt: P00749

Pathways: Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy, Smooth Muscle Cell Migration