CD40 Ligand Kit ELISA

N° du produit ABIN411377

Détail du produit CD40 Ligand Kit ELISA

Antigène: CD40 Ligand (CD40LG)

Épitope: AA 108-261

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5-4000 pg/mL

Seuil minimal de détection: 62.5 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human soluble CD40L

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli
Immunogen sequence: E108-L261

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <15pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: E.coli
Immunogen sequence: E108-L261

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Belongs to the tumor necrosis factor family.

Tissue Specificity: Specifically expressed on activated CD4+ T- lymphocytes.

Plaque: Pre-coated

Protocole: human CD40L ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD40L has been precoated onto 96-well plates. Standards(E.coli, E108-L261) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD40L is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD40L amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human CD40L standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CD40L standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(ng/ml): 0.44, Standard deviation: 0.026, CV(%): 5.9
• Sample 2: n=16, Mean(ng/ml): 1.26, Standard deviation: 0.064, CV(%): 5.1
• Sample 3: n=16, Mean(ng/ml): 2.38, Standard deviation: 0.145, CV(%): 6.1,
• Sample 1: n=24, Mean(ng/ml): 0.42, Standard deviation: 0.029, CV(%): 6.8
• Sample 2: n=24, Mean(ng/ml): 1.41, Standard deviation: 0.086, CV(%): 6.1
• Sample 3: n=24, Mean(ng/ml): 2.46, Standard deviation: 0.182, CV(%): 7.4

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour CD40 Ligand Kit ELISA (ABIN411377)

Portillo, Corcino, Miao, Tang, Sheibani, Kern, Dubyak, Subauste: "CD40 in Retinal Müller Cells Induces P2X7-Dependent Cytokine Expression in Macrophages/Microglia in Diabetic Mice and Development of Early Experimental Diabetic Retinopathy." dans: Diabetes, (2016) (PubMed).

Détail du antigène

Antigène: CD40 Ligand (CD40LG)

Autre désignation: CD40LG (CD40LG Produits)

Synonymes: CD154 Kit ELISA, cd40l Kit ELISA, CD40L Kit ELISA, HIGM1 Kit ELISA, IGM Kit ELISA, IMD3 Kit ELISA, T-BAM Kit ELISA, TNFSF5 Kit ELISA, TRAP Kit ELISA, gp39 Kit ELISA, hCD40L Kit ELISA, CD40-L Kit ELISA, Cd40l Kit ELISA, Ly-62 Kit ELISA, Ly62 Kit ELISA, Tnfsf5 Kit ELISA, CD40 ligand Kit ELISA, TNF superfamily member 5 Kit ELISA, CD40LG Kit ELISA, cd40l Kit ELISA, Cd40lg Kit ELISA

Sujet:

Protein Function: Mediates B-cell proliferation in the absence of co- stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. .

Background: CD40 ligand(CD40L) is a type II membrane protein of 261 amino acids on activated T cells that induces B cell proliferation and immunoglobulin secretion. It has homology with tumour necrosis factor-alpha and -beta, and has important functions in B-cell activation and differentiation. Human CD40L with 5 exons, is mapped to the proximal region of the mouse X chromosome on Xq26.3-27.1, and can be detected on T cells but is absent from B cells and monocytes. Since CD40L is expressed on platelets and released from them on activation, its predictive value as a marker for clinical outcome and the therapeutic effect of inhibition of glycoprotein IIb/IIIa receptor in patients with acute coronary syndromes was investigated. The soluble CD40L may be involved in the process of restenosis and that it exerts its effect by triggering a complex group of inflammatory reactions on endothelial and mononuclear cells.CD40L plays a central role in the pathophysiology of acute coronary syndromes, and has a role in the pathogenesis of coronary artery lesions.

Synonyms: CD40 ligand,CD40-L,T-cell antigen Gp39,TNF-related activation protein,TRAP,Tumor necrosis factor ligand superfamily member 5,CD154,CD40 ligand, membrane form,CD40 ligand, soluble form,CD40LG,CD40L, TNFSF5, TRAP,

Full Gene Name: CD40 ligand

Cellular Localisation: Cell membrane, Single-pass type II membrane protein.

ID gène: 959

UniProt: P29965

Pathways: Signalisation NF-kappaB, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints