P-Cadherin Kit ELISA

N° du produit ABIN411390

Détail du produit P-Cadherin Kit ELISA

Antigène: P-Cadherin (CDH3)

Épitope: AA 108-654

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5-4000 pg/mL

Seuil minimal de détection: 62.5 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human P-Cadherin

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: D108-G654

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <2pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: D108-G654

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Contains 5 cadherin domains.

Tissue Specificity: Expressed in some normal epithelial tissues and in some carcinoma cell lines. .

Plaque: Pre-coated

Protocole: human P-Cadherin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for P-Cadherin has been precoated onto 96-well plates. Standards(NSO, D108-G654) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for P-Cadherin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human P-Cadherin amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human P-Cadherin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human P-Cadherin standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 426, Standard deviation: 27.69, CV(%): 6.5
• Sample 2: n=16, Mean(pg/ml): 1211, Standard deviation: 82.35, CV(%): 6.8
• Sample 3: n=16, Mean(pg/ml): 2574, Standard deviation: 185.33, CV(%): 7.2,
• Sample 1: n=24, Mean(pg/ml): 432, Standard deviation: 32.83, CV(%): 7.6
• Sample 2: n=24, Mean(pg/ml): 1221, Standard deviation: 96.46, CV(%): 7.9
• Sample 3: n=24, Mean(pg/ml): 2624, Standard deviation: 233.04, CV(%): 8.5

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: P-Cadherin (CDH3)

Autre désignation: CDH3 (CDH3 Produits)

Sujet:

Protein Function: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells, cadherins may thus contribute to the sorting of heterogeneous cell types.

Background: Cadherins are calcium-dependent cell-cell adhesion molecules that mediate cell-cell binding in a homophilic manner. They play an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. Cadherin expression is regulated spatially as well as temporally. Cadherins are thought to play an important role in development and maintenance of tissues through selective cell-cell adhesion activity and may be involved also in the invasion and metastasis of malignant tumors. Cadherin regulates dendritic spine morphogenesis. A cadherin gene cluster is mapped to a region of chromosome 5 subject to frequent allelic loss in carcinoma. The standard product used in this kit is recombinant P-Cadherin with the molecular mass of 120-130Kda after glycosylation.

Synonyms: Cadherin-3,Placental cadherin,P-cadherin,CDH3,CDHP,

Full Gene Name: Cadherin-3

Cellular Localisation: Cell membrane, Single-pass type I membrane protein.

ID gène: 1001

UniProt: P22223