Cathepsin B Kit ELISA

N° du produit ABIN411391

Détail du produit Cathepsin B Kit ELISA

Antigène: Cathepsin B (CTSB)

Épitope: AA 18-339

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156-10000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Cathepsin B

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: R18-I339

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <5pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: R18-I339

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Belongs to the peptidase C1 family.

Plaque: Pre-coated

Protocole: human Cathepsin B ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Cathepsin B has been precoated onto 96-well plates. Standards(NSO, R18-I339) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Cathepsin B is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Cathepsin B amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Cathepsin B standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Cathepsin B standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 1350, Standard deviation: 68.9, CV(%): 5.1
• Sample 2: n=16, Mean(pg/ml): 3080, Standard deviation: 150.9, CV(%): 4.9
• Sample 3: n=16, Mean(pg/ml): 6170, Standard deviation: 357.9, CV(%): 5.8,
• Sample 1: n=24, Mean(pg/ml): 1452, Standard deviation: 113.3, CV(%): 7.8
• Sample 2: n=24, Mean(pg/ml): 3209, Standard deviation: 256.7, CV(%): 8
• Sample 3: n=24, Mean(pg/ml): 6124, Standard deviation: 520.5, CV(%): 8.5

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: Cathepsin B (CTSB)

Autre désignation: CTSB (CTSB Produits)

Synonymes: APPS Kit ELISA, CPSB Kit ELISA, CB Kit ELISA, apps Kit ELISA, cpsb Kit ELISA, LOC692390 Kit ELISA, CTSB Kit ELISA, cathepsin B Kit ELISA, cathepsin B S homeolog Kit ELISA, CTSB Kit ELISA, Ctsb Kit ELISA, ctsb.S Kit ELISA, LOC692390 Kit ELISA

Sujet:

Protein Function: Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.

Background: Cathepsin B is an enzymatic protein belonging to the peptidase or protease families. In humans, it is coded by the CTSB gene.1, 2 And this gene is mapped to chromosome 8p22.3 The protein encoded by this gene is a lysosomal cysteine proteinase composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is a member of the peptidase C1 family. Cathepsin B was once suspected as a candidate protease participating in the conversion of beta-amyloid precursor protein into the amyloid plaques found in Alzheimer's disease patients. However, this function is now known to be mediated by BACE1 protease. It is now thought that cathepsin B can degrade beta-amyloid precursor protein into harmless fragments. Thus, it is conceivable cathepsin B may play a pivotal role in the natural defense against Alzheimer's disease.4 Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors. At least five transcript variants encoding the same protein have been found for this gene. The standard product used in this kit is recombinant human Cathepsin B with the molecular mass of 37KDa.

Synonyms: Cathepsin B,3.4.22.1,APP secretase,APPS,Cathepsin B1,Cathepsin B light chain,Cathepsin B heavy chain,CTSB,CPSB,

Full Gene Name: Cathepsin B

Cellular Localisation: Lysosome. Melanosome. Secreted, extracellular space . Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

ID gène: 1508

UniProt: P07858

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades