CXCL10 Kit ELISA

N° du produit ABIN411396

Détail du produit CXCL10 Kit ELISA

Antigène: CXCL10 (Chemokine (C-X-C Motif) Ligand 10 (CXCL10))

Épitope: AA 22-98

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2-2000 pg/mL

Seuil minimal de détection: 31.2 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CXCL10/IP-10

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin)

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli
Immunogen sequence: I22-P98

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <1pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: E.coli
Immunogen sequence: I22-P98

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Tissue Specificity: Expressed in the spleen, thymus, lymph nodes and liver. .

Plaque: Pre-coated

Protocole: mouse CXCL10 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CXCL10 has been precoated onto 96-well plates. Standards(E.coli, I22-P98) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL10 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CXCL10 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse CXCL10 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma( heparin) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse CXCL10 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 112, Standard deviation: 5.82, CV(%): 5.2
• Sample 2: n=16, Mean(pg/ml): 259, Standard deviation: 14.5, CV(%): 5.6
• Sample 3: n=16, Mean(pg/ml): 973, Standard deviation: 70.1, CV(%): 7.2,
• Sample 1: n=24, Mean(pg/ml): 125, Standard deviation: 12.25, CV(%): 9.8
• Sample 2: n=24, Mean(pg/ml): 276, Standard deviation: 22.4, CV(%): 8.1
• Sample 3: n=24, Mean(pg/ml): 1093, Standard deviation: 84.2, CV(%): 7.7

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour CXCL10 Kit ELISA (ABIN411396)

Tomita, Kohli, MacLaurin, Hirsova, Guo, Sanchez, Gelbard, Blaxall, Ibrahim: "Mixed-lineage kinase 3 pharmacological inhibition attenuates murine nonalcoholic steatohepatitis." dans: JCI insight, Vol. 2, Issue 15, (2017) (PubMed).

Liu, An, Liu, Li, Tang, Chen: "Mouse lung slices: An ex vivo model for the evaluation of antiviral and anti-inflammatory agents against influenza viruses." dans: Antiviral research, Vol. 120, pp. 101-11, (2015) (PubMed).

Détail du antigène

Antigène: CXCL10 (Chemokine (C-X-C Motif) Ligand 10 (CXCL10))

Autre désignation: CXCL10 (CXCL10 Produits)

Synonymes: CXCL10 Kit ELISA, C7 Kit ELISA, IFI10 Kit ELISA, INP10 Kit ELISA, IP-10 Kit ELISA, SCYB10 Kit ELISA, crg-2 Kit ELISA, gIP-10 Kit ELISA, mob-1 Kit ELISA, CRG-2 Kit ELISA, IP10 Kit ELISA, Ifi10 Kit ELISA, Scyb10 Kit ELISA, C-X-C motif chemokine ligand 10 Kit ELISA, C-X-C motif chemokine 10 Kit ELISA, chemokine (C-X-C motif) ligand 10 Kit ELISA, CXCL10 Kit ELISA, cxl10 Kit ELISA, Cxcl10 Kit ELISA

Sujet:

Protein Function: In addition to its role as a proinflammatory cytokine, may participate in T-cell effector function and perhaps T-cell development.

Background: Chemokine(C-X-C motif) ligand 10(CXCL10) or IP-10 is a small cytokine belonging to the CXC chemokine family that is also known as 10 kDa interferon-gamma-induced protein(gamma-IP10 or IP-10). CXCL10 is secreted by several cell types in response to IFN-gamma. These cell types include monocytes, endothelial cells and fibroblasts. CXCL10 has been attributed to several roles, such as chemoattraction for monocytes/macrophages, T cells, NK cells, and dendritic cells, promotion of T cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation and angiogenesis.It is a potent inhibitor of angiogenesis in vivo. The gene for CXCL10 is located on human chromosome 4 in a cluster among several other CXC chemokines. It is a RAS target gene and is overexpressed in the majority of colorectal cancers.This chemokine elicits its effects by binding to the cell surface chemokine receptor CXCR3.

Synonyms: C-X-C motif chemokine 10,10 kDa interferon gamma-induced protein,Gamma-IP10,IP-10,C7,Interferon-gamma induced protein CRG-2,Small-inducible cytokine B10,Cxcl10,Crg2, Ifi10, Inp10, Scyb10,

Full Gene Name: C-X-C motif chemokine 10

Cellular Localisation: Secreted.

ID gène: 15945

UniProt: P17515