IgG Kit ELISA
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- Antigène Voir toutes IgG Kits ELISA
- IgG
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Reactivité
- Rat
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Seuil minimal de détection
- 156 ng/mL
- Application
- ELISA
- Fonction
- The IgG(rat)-ELISA is designed for the quantitative determination of rat IgG in complex samples (serum or other biological samples).
- Type d'échantillon
- Serum
- Analytical Method
- Quantitative
- Attributs du produit
- The determination of rat IgG is carried out as direct sandwich ELISA.
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- Discover our top product IgG Kit ELISA
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- Plaque
- Pre-coated
- Protocole
- An antibody specific for rat IgG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgG present is bound. After washing away any unbound substances, an enzyme-linked antibody is added. Following a wash, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is proportional to the IgG concentration.
- Procédure de l'essai
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Incubation of samples/standards: 100 µL/well 1 hr with shaking or 2 hrs without shaking
Wash Incubation of detection antibody: 100 µL/well 1 hr with shaking or 2 hrs without shaking
Wash Substrate incubation: 100 µL/well 10 min, room temperature
Add stop solution: 100 µL/well
Read at 450 nm - Précision du teste
- Intraassay precision (CV): (n=10) at 73 ng/ml: 5.8% at 274 ng/ml: 8.9%
- Restrictions
- For Research Use only
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- Stock
- 4 °C
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- Antigène Voir toutes IgG Kits ELISA
- IgG
- Abstract
- IgG Produits
- Classe de substances
- Antibody
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