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EIF2AK2 Kit ELISA

EIF2AK2 Reactivité: Souris Colorimetric Competition ELISA Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN454366
  • Antigène Voir toutes EIF2AK2 Kits ELISA
    EIF2AK2 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2))
    Reactivité
    • 5
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Competition ELISA
    Application
    ELISA
    Fonction
    This immunoassay kit allows for the in vitro quantitative determination of mouse Anti-nuclear Antibody, ANA concentrations in cell culture supernates, serum, plasma and other biological fluids.
    Type d'échantillon
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    Specificité
    This assay recognizes recombinant and natural mouse ANA.
    Réactivité croisée (Details)
    No significant cross-reactivity or interference was observed.
    Sensibilité
    < 0.78 U/L
    The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.
    Attributs du produit
    Mus musculus,Mouse,Interferon-induced, double-stranded RNA-activated protein kinase,Eukaryotic translation initiation factor 2-alpha kinase 2,eIF-2A protein kinase 2,Interferon-inducible RNA-dependent
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  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    The microtiter plate provided in this kit has been pre-coated with an antigen specific to ANA. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antigen preparation specific for ANA and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain ANA, biotin-conjugated antigen and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ANA in the samples is then determined by comparing the O.D. 2 of the samples to the standard curve.
    Restrictions
    For Research Use only
  • Stock
    4 °C/-20 °C
    Stockage commentaire
    The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
  • Antigène Voir toutes EIF2AK2 Kits ELISA
    EIF2AK2 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2))
    Autre désignation
    Eif2ak2 (EIF2AK2 Produits)
    Synonymes
    PKR Kit ELISA, pkr Kit ELISA, EIF2AK2 Kit ELISA, DKFZp469K1934 Kit ELISA, EIF2AK1 Kit ELISA, PRKR Kit ELISA, 2310047A08Rik Kit ELISA, 4732414G15Rik Kit ELISA, AI467567 Kit ELISA, AI747578 Kit ELISA, Pkr Kit ELISA, Prkr Kit ELISA, Tik Kit ELISA, eukaryotic translation initiation factor 2 alpha kinase 2 Kit ELISA, eukaryotic translation initiation factor 2-alpha kinase 2 Kit ELISA, EIF2AK2 Kit ELISA, eif2ak2 Kit ELISA, Eif2ak2 Kit ELISA
    Classe de substances
    Antibody
    Sujet
    Anti-nuclear antibodies (ANAs, also known as anti-nuclear factor or ANF) are antibodies directed against contents of the cell nucleus.They are present in higher than normal numbers in autoimmune disease. The ANA test measures the pattern and amount of autoantibody which can attack the body's tissues as if they were foreign material. Autoantibodies are present in low titers in the general population, but in about 5% of the population, their concentration is increased, and about half of this 5% have an autoimmune disease. Normal titer of ANA is 1:40. Higher titers are indicative of an autoimmune disease. The presence of ANA is indicative of lupus erythematosus (present in 80-90% of cases), though they also appear in some other auto-immune diseases such as Sj ö gren's syndrome (60%), rheumatoid arthritis, autoimmune hepatitis, scleroderma and polymyositis & dermatomyositis (30%), and various non-rheumatological conditions associated with tissue damage. ANA are also directed to the nuclear pore complex in primary biliary cirrhosis. Other conditions with high ANA titre include Addison disease, Idiopathic thrombocytopenic purpura (ITP), Hashimoto's, Autoimmune hemolytic anemia, Type I diabetes mellitus, Mixed connective tissue disorder.
    Pathways
    Réparation de l'ADN, ER-Nucleus Signaling, Hepatitis C
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