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Titin Kit ELISA

TTN Reactivité: Humain Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
N° du produit ABIN454416
  • Antigène Voir toutes Titin (TTN) Kits ELISA
    Titin (TTN)
    Reactivité
    • 3
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.312 ng/mL - 20 ng/mL
    Seuil minimal de détection
    0.312 ng/mL
    Application
    ELISA
    Fonction
    This immunoassay kit allows for the in vitro quantitative determination of human Titin , concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates.
    Type d'échantillon
    Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
    Analytical Method
    Quantitative
    Specificité
    This assay recognizes recombinant and natural human Anti-titin Ab.
    Réactivité croisée (Details)
    No significant cross-reactivity or interference was observed.
    Sensibilité
    0.138 ng/mL
    Attributs du produit
    Homo sapiens,Human,Titin,Connectin,Rhabdomyosarcoma antigen MU-RMS-40.14,TTN,2.7.11.1
    Ingrédients
    Reagent (Quantity): Assay plate (1), Standard (2), Sample Diluent (1x20ml), Assay Diluent A (1x10ml), Assay DiluentB 1 x 10ml Detection Reagent A (1x120µl), Detection Reagent B (1x120µl), Wash Buffer(25 x concentrate) (1x30ml), Substrate (1x10ml), Stop Solution (1x10ml)
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  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Préparation des réactifs

    Bring all reagents to room temperature before use. Wash Buffer - If crystals have formed in the concentrate, warm to room temperature and mix gently until the crystals have completely dissolved. Dilute 30 mL of Wash Buffer Concentrate into deionized or distilled water to prepare 750 mL of Wash Buffer. Standard - Reconstitute the Standard with 1.0mL of Sample Diluent. This reconstitution produces a stock solution of 20ng/mL. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making serial dilutions (Making serial dilution in the wells directly is not permitted). The undiluted standard serves as the high standard (20ng/mL) The Sample Diluent serves as the zero standard (0ng/mL). Detection Reagent A and B - Dilute to the working concentration using Assay Diluent A and B (1:100), respectively.

    Prélèvement de l'échantillon
    Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 x g. Remove serum and assay immediately or aliquot and store samples at -20 °C or -80 °C. Plasma - Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 1000 x g at 2 - 8 °C within 30 minutes of collection. Store samples at -20 °C or -80° 2 C. Avoid repeated freeze-thaw cycles. Other biological fluids - Remove particulates by centrifugation and assay immediately or aliquot and store samples at -20 °C or -80 °C. Avoid repeated freeze-thaw cycles. Note: Serum, plasma, and cell culture supernatant samples to be used within 7 days may be stored at 2-8C, otherwise samples must stored at -20 °C (≤ 3 months) or -80 °C (≤ 6 months) to avoid loss of bioactivity and contamination. Avoid freeze-thaw cycles. When performing the assay slowly bring samples to room temperature. It is recommended that all samples be assayed in duplicate.
    Calcul des résultats

    Average the duplicate readings for each standard, control, and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the y-axis against the concentration on the x-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the ANTI-TITIN AB concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.

    Précision du teste
    Intra-AssayCV: < 3.9%Inter-AssayCV: < 6.2%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    1. The kit should not be used beyond the expiration date on the kit label.
    2. Do not mix or substitute reagents with those from other lots or sources.
    3. If samples generate values higher than the highest standard, further dilute the samples with the Assay Diluent and repeat the assay. Any variation in standard diluent, operator, pipetting technique, washing technique,incubation time or temperature, and kit age can cause variation in binding.
    4. This assay is designed to eliminate interference by soluble receptors, ligands, binding proteins, and other factors present in biological samples. Until all factors have been tested in the Immunoassay, the possibility of interference cannot be excluded.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
  • Antigène Voir toutes Titin (TTN) Kits ELISA
    Titin (TTN)
    Autre désignation
    TTN (TTN Produits)
    Synonymes
    CMD1G Kit ELISA, CMH9 Kit ELISA, CMPD4 Kit ELISA, EOMFC Kit ELISA, HMERF Kit ELISA, LGMD2J Kit ELISA, MYLK5 Kit ELISA, TMD Kit ELISA, 1100001C23Rik Kit ELISA, 2310036G12Rik Kit ELISA, 2310057K23Rik Kit ELISA, 2310074I15Rik Kit ELISA, AF006999 Kit ELISA, AV006427 Kit ELISA, D330041I19Rik Kit ELISA, D830007G01Rik Kit ELISA, L56 Kit ELISA, mdm Kit ELISA, shru Kit ELISA, UNC-22 Kit ELISA, titin Kit ELISA, TTN Kit ELISA, Ttn Kit ELISA, titin Kit ELISA, TTNLOC100620261 Kit ELISA
    Classe de substances
    Antibody
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