Fibronectin 1 Kit ELISA

N° du produit ABIN455123

Détail du produit Fibronectin 1 Kit ELISA

Antigène: Fibronectin 1 (FN1)

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.78-50 ng/mL

Seuil minimal de détection: 0.78 ng/mL

Application: ELISA

Fonction: This immunoassay kit allows for the in vitro quantitative determination of mouse Fibronectin,FN concentrations in cell culture supernates, serum, plasma and other biological fluids.

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificité: This assay recognizes recombinant and natural mouse FN.

Réactivité croisée (Details): No significant cross-reactivity or interference was observed.

Sensibilité: < 11.5 ng/mL
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.

Attributs du produit: Mus musculus,Mouse,Fibronectin,FN,Fn1

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FN. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for FN and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain FN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of FN in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Stockage commentaire: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Détail du antigène

Antigène: Fibronectin 1 (FN1)

Autre désignation: Fn1 (FN1 Produits)

Synonymes: FN1 Kit ELISA, FN Kit ELISA, cig Kit ELISA, fibronectin Kit ELISA, finc Kit ELISA, lets Kit ELISA, msf Kit ELISA, Fn Kit ELISA, fn2 Kit ELISA, fb80d10 Kit ELISA, wu:fb80d10 Kit ELISA, CIG Kit ELISA, ED-B Kit ELISA, FINC Kit ELISA, FNZ Kit ELISA, GFND Kit ELISA, GFND2 Kit ELISA, LETS Kit ELISA, MSF Kit ELISA, E330027I09 Kit ELISA, Fn-1 Kit ELISA, FIBNEC Kit ELISA, fn-1 Kit ELISA, fibronectin 1 Kit ELISA, fibronectin 1a Kit ELISA, fibronectin 1 S homeolog Kit ELISA, FN1 Kit ELISA, fn1 Kit ELISA, fn1a Kit ELISA, Fn1 Kit ELISA, fn1.S Kit ELISA

Pathways: Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy