SFRP2 Kit ELISA
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- Antigène Voir toutes SFRP2 Kits ELISA
- SFRP2 (Secreted Frizzled-Related Protein 2 (SFRP2))
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Reactivité
- Souris
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 0.78-50 ng/mL
- Seuil minimal de détection
- 0.78 ng/mL
- Application
- ELISA
- Fonction
- This immunoassay kit allows for the in vitro quantitative determination of mouse Free-T3 concentrations in cell culture supernates, serum, plasma and other biological fluids.
- Type d'échantillon
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- Specificité
- This assay recognizes recombinant and natural mouse Free-T3.
- Réactivité croisée (Details)
- No significant cross-reactivity or interference was observed.
- Sensibilité
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< 0.078 pmol/L
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero. - Attributs du produit
- Mus musculus,Mouse,Secreted frizzled-related protein 2,sFRP-2,Protein SDF5,Secreted apoptosis-related protein 1,SARP-1,Sfrp2,Sarp1,Sdf5
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- Discover our best selling SFRP2 Kit ELISA
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- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Protocole
- The microtiter plate provided in this kit has been pre-coated with an antibody specific to Free-T3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Free-T3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Free-T3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Free-T3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Restrictions
- For Research Use only
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- Stock
- 4 °C/-20 °C
- Stockage commentaire
- The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
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- Antigène Voir toutes SFRP2 Kits ELISA
- SFRP2 (Secreted Frizzled-Related Protein 2 (SFRP2))
- Autre désignation
- Sfrp2 (SFRP2 Produits)
- Synonymes
- frp-2 Kit ELISA, sarp1 Kit ELISA, sdf-5 Kit ELISA, sfrp-2 Kit ELISA, SFRP2 Kit ELISA, sfrp2 Kit ELISA, FRP-2 Kit ELISA, SARP1 Kit ELISA, SDF-5 Kit ELISA, AI851596 Kit ELISA, Sdf5 Kit ELISA, hm:zeh0225 Kit ELISA, zeh0225 Kit ELISA, zgc:153618 Kit ELISA, SFRP-2 Kit ELISA, secreted frizzled-related protein 2 Kit ELISA, secreted frizzled related protein 2 Kit ELISA, secreted frizzled-related protein 2 S homeolog Kit ELISA, secreted frizzled-related protein Kit ELISA, sfrp2 Kit ELISA, sfrp2 Kit ELISA, SFRP2 Kit ELISA, sfrp2.S Kit ELISA, LOAG_01461 Kit ELISA, Sfrp2 Kit ELISA
- Pathways
- Signalisation WNT, Tube Formation, Positive Regulation of Endopeptidase Activity, Negative Regulation of intrinsic apoptotic Signaling, Positive Regulation of fat Cell Differentiation
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