TNFSF8 Kit ELISA

N° du produit ABIN456421

Détail du produit TNFSF8 Kit ELISA

Antigène: TNFSF8 (Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8))

Épitope: Soluble

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156-10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Fonction: This immunoassay kit allows for the specific measurement of human sCD30L concentrations in cell culture supernates, serum, and plasma.

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma

Analytical Method: Quantitative

Specificité: This assay recognizes recombinant and natural human sCD30L.

Réactivité croisée (Details): No significant cross-reactivity or interference was observed.

Attributs du produit: Homo sapiens,TNFSF8,Tumor necrosis factor ligand superfamily member 8,CD30 ligand,CD30-L,TNFSF8,CD30L,CD30LG,CD153,CD153

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for sCD30L has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any sCD30L present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for sCD30L is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sCD30L bound in the initial step. The color development is stopped and the intensity of the color is measured.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Stockage commentaire: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Détail du antigène

Antigène: TNFSF8 (Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8))

Autre désignation: TNFSF8 (TNFSF8 Produits)

Synonymes: CD153 Kit ELISA, CD30LG Kit ELISA, Cd30l Kit ELISA, CD30L Kit ELISA, TNFRSF8 Kit ELISA, TNFSF8 Kit ELISA, tumor necrosis factor (ligand) superfamily, member 8 Kit ELISA, TNF superfamily member 8 Kit ELISA, tumor necrosis factor superfamily member 8 Kit ELISA, Tnfsf8 Kit ELISA, TNFSF8 Kit ELISA

Sujet: The human CD30 ligand, CD153, is a 40kDa Type II transmembrane glycoprotein. It interacts with its receptor, CD30, as a homotrimer Soluble trimeric CD153 binds to membrane-anchored CD30 with a relatively high affinity and is very effective in triggering cell death and TNFα. CD153 is a member of the TNF superfamily which is primarily expressed by activated T cells, B cells and monocytes. Its function in programmed cell death has been proven, as well as its involvement in disorders like a variety of lymphoid malignancies like Hodgkin’s disease and anaplastic large cell lymphoma.