NUCB2 Kit ELISA

N° du produit ABIN4881312

Détail du produit NUCB2 Kit ELISA

Antigène: NUCB2 (NES1) (Nesfatin-1 (NES1))

Reactivité: Humain, Rat, Souris

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Gamme de detection: 0.1-1.000 ng/mL

Seuil minimal de détection: 0.1 ng/mL

Application: ELISA

Fonction: Human/Mouse/Rat Nesfatin EIA Kit optimized for serum and cell culture supernatants. Competition-based ELISA on a 96-well strip plate.

Type d'échantillon: Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: This kit detects human, mouse and rat 82aa Nesfatin protein. No other active isoforms have been reported.

Sensibilité: 400 pg/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Standard Peptide
• Assay Diluent(s)
• Biotinylated Peptide
• HRP-Streptavidin
• TMB One-Step Substrate
• Stop Solution
• Assay Diagram
• Positive Control Sample
• Capture Antibody
• User Manual

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Orbital shaker
• Aluminum foil
• Saran Wrap
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• SigmaPlot software (or other software that can perform four-parameter logistic regression models)

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samplesHuman: 2X / Mouse: 2X / Rat: 2X

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL detection antibody to each well.
3. Incubate 1.5 h at RT or O/N at 4 °C.
4. Add 100 μL standard or sample to each well.
5. Incubate 2.5 h at RT.
6. Add 100 μL prepared streptavidin solution.
7. Incubate 45 min at RT.
8. Add 100 μL TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL Stop Solution to each well.
11. Read plate at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.

Date de péremption: 6 months

Références pour NUCB2 Kit ELISA (ABIN4881312)

Serin, Bakacak, Ercan, Köstü, Avci, Arıkan, Kıran: "The evaluation of Nesfatin-1 levels in patients with and without intrauterine growth restriction." dans: The journal of maternal-fetal & neonatal medicine, Vol. 29, Issue 9, pp. 1409-13, (2016) (PubMed).

Abaci, Catli, Anik, Kume, Bober: "The relation of serum nesfatin-1 level with metabolic and clinical parameters in obese and healthy children." dans: Pediatric diabetes, Vol. 14, Issue 3, pp. 189-95, (2013) (PubMed).

Détail du antigène

Antigène: NUCB2 (NES1) (Nesfatin-1 (NES1))

Autre désignation: Nesfatin (NES1 Produits)

Synonymes: nucleobindin-2 Kit ELISA, nucleobindin 2 Kit ELISA, NUCB2 Kit ELISA, Nucb2 Kit ELISA

ID gène: 4925

UniProt: P80303