Substance P Kit ELISA

N° du produit ABIN4881333

Détail du produit Substance P Kit ELISA

Antigène: Substance P

Reactivité: Humain, Souris, Rat

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Gamme de detection: 0.1-1.000 ng/mL

Seuil minimal de détection: 0.1 ng/mL

Application: ELISA

Fonction: Human/Mouse/Rat Substance P EIA Kit optimized for serum, plasma, and cell culture supernatants. Competition-based ELISA on a 96-well strip plate.

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: This EIA kit is designed to detect human, mouse, and rat Substance P.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Standard Peptide
• Assay Diluent(s)
• Biotinylated Peptide
• HRP-Streptavidin
• TMB One-Step Substrate
• Stop Solution
• Assay Diagram
• Positive Control Sample
• Capture Antibody
• User Manual

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Orbital shaker
• Aluminum foil
• Saran Wrap
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• SigmaPlot software (or other software that can perform four-parameter logistic regression models)

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samplesHuman: 2x / Mouse: 8x / Rat: 8X

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL detection antibody to each well.
3. Incubate 1.5 h at RT or O/N at 4 °C.
4. Add 100 μL standard or sample to each well.
5. Incubate 2.5 h at RT.
6. Add 100 μL prepared streptavidin solution.
7. Incubate 45 min at RT.
8. Add 100 μL TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL Stop Solution to each well.
11. Read plate at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.

Date de péremption: 6 months

Détail du antigène

Antigène: Substance P

Abstract: Substance P Produits

Synonymes: Hs.2563 Kit ELISA, NK2 Kit ELISA, NKNA Kit ELISA, NPK Kit ELISA, TAC2 Kit ELISA, PPTA3 Kit ELISA, Ppt5fl Kit ELISA, RATPPTA3 Kit ELISA, TAC Kit ELISA, 4930528L02Rik Kit ELISA, NK-1 Kit ELISA, NK1 Kit ELISA, Nkna Kit ELISA, PPT-A Kit ELISA, PPTA Kit ELISA, SP Kit ELISA, PPT Kit ELISA, beta-PPT-A Kit ELISA, NKA Kit ELISA, tachykinin precursor 1 Kit ELISA, tachykinin, precursor 1 Kit ELISA, tachykinin 1 Kit ELISA, TAC1 Kit ELISA, Tac1 Kit ELISA

ID gène: 6863

UniProt: P20366