2B4 Kit ELISA

N° du produit ABIN4881695

Détail du produit 2B4 Kit ELISA

Antigène: 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.05-12 ng/mL

Seuil minimal de détection: 0.05 ng/mL

Application: ELISA

Fonction: Human 2B4 (CD244) ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: The antibody pair provided in this kit recognizes human 2B4/CD244/SLAMF4.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples2 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))

Autre désignation: 2B4 (CD244 Produits)

Synonymes: CD244 Kit ELISA, 2B4 Kit ELISA, NAIL Kit ELISA, NKR2B4 Kit ELISA, Nmrk Kit ELISA, SLAMF4 Kit ELISA, C9.1 Kit ELISA, F730046O15Rik Kit ELISA, Ly90 Kit ELISA, CD244 molecule Kit ELISA, CD244 natural killer cell receptor 2B4 Kit ELISA, CD244 Kit ELISA, Cd244 Kit ELISA

ID gène: 51744

UniProt: Q9BZW8