AHCY Kit ELISA

N° du produit ABIN4881774

Détail du produit AHCY Kit ELISA

Antigène: AHCY (Adenosylhomocysteinase (AHCY))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human AHCY ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human APE1.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: AHCY (Adenosylhomocysteinase (AHCY))

Autre désignation: AHCY (AHCY Produits)

Synonymes: sahh Kit ELISA, PSPTO5068 Kit ELISA, Ahcy Kit ELISA, SAHH Kit ELISA, adoHcyase Kit ELISA, ahcy Kit ELISA, AA987153 Kit ELISA, AL024110 Kit ELISA, CuBP Kit ELISA, D150 Kit ELISA, cb1079 Kit ELISA, hm:zeh1173 Kit ELISA, hm:zeh1364 Kit ELISA, wu:fj67b02 Kit ELISA, adenosylhomocysteinase Kit ELISA, Adenosylhomocysteinase Kit ELISA, S-adenosylhomocysteine hydrolase Kit ELISA, adenosylhomocysteinase S homeolog Kit ELISA, AHCY Kit ELISA, ahcy Kit ELISA, ahcY Kit ELISA, MMAH_RS02605 Kit ELISA, Srot_2217 Kit ELISA, Ndas_3755 Kit ELISA, Deba_0936 Kit ELISA, Palpr_2392 Kit ELISA, Calni_0569 Kit ELISA, LOC100282150 Kit ELISA, Intca_2510 Kit ELISA, Marky_0392 Kit ELISA, Desac_1394 Kit ELISA, Tc00.1047053511229.50 Kit ELISA, Tc00.1047053511589.200 Kit ELISA, Tb11.01.1350 Kit ELISA, LMJF_36_3910 Kit ELISA, MCYG_06244 Kit ELISA, Ahcy Kit ELISA, ahcy.S Kit ELISA

ID gène: 191

UniProt: P23526