AIF Kit ELISA

N° du produit ABIN4881777

Détail du produit AIF Kit ELISA

Antigène: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human AIF ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human B7-H4.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Autre désignation: AIF (AIFM1 Produits)

Synonymes: AIF Kit ELISA, CMTX4 Kit ELISA, COWCK Kit ELISA, COXPD6 Kit ELISA, PDCD8 Kit ELISA, CG7263 Kit ELISA, DmAIF Kit ELISA, Dmel\\CG7263 Kit ELISA, GB16024 Kit ELISA, DDBDRAFT_0187853 Kit ELISA, DDBDRAFT_0191137 Kit ELISA, DDB_0187853 Kit ELISA, DDB_0191137 Kit ELISA, aif Kit ELISA, pdcd8 Kit ELISA, AIFM1 Kit ELISA, PCD8 Kit ELISA, AIFsh2 Kit ELISA, Hq Kit ELISA, Pdcd8 Kit ELISA, mAIF Kit ELISA, Aif Kit ELISA, zgc:91994 Kit ELISA, apoptosis inducing factor mitochondria associated 1 Kit ELISA, allograft inflammatory factor 1 Kit ELISA, Apoptosis inducing factor Kit ELISA, apoptosis-inducing factor 1, mitochondrial Kit ELISA, apoptosis inducing factor Kit ELISA, apoptosis inducing factor, mitochondria associated 1 Kit ELISA, apoptosis-inducing factor, mitochondrion-associated, 1 Kit ELISA, apoptosis-inducing factor, mitochondrion-associated 1 Kit ELISA, AIFM1 Kit ELISA, AIF1 Kit ELISA, AIF Kit ELISA, LOC412212 Kit ELISA, aif Kit ELISA, aifm1 Kit ELISA, Aifm1 Kit ELISA

ID gène: 9131

UniProt: O95831

Pathways: Apoptose, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, L'effet Warburg