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AKR1C1 Kit ELISA
DDH
Reactivité: Humain
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4881783
Détail du produit AKR1C1 Kit ELISA
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Antigène
Voir toutes AKR1C1 (DDH) Kits ELISA
AKR1C1 (DDH)
(Dihydrodiol Dehydrogenase (DDH))
Reactivité
Humain
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Fonction
Custom Human AKR1C1 ELISA Kit.
Type d'échantillon
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificité
This ELISA antibody pair recognizes Human BCHE.
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Information d'application
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Volume d'échantillon
100 μL
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes AKR1C1 (DDH) Kits ELISA
AKR1C1 (DDH)
(Dihydrodiol Dehydrogenase (DDH))
Autre désignation
AKR1C1 (DDH Produits )
Synonymes
2-ALPHA-HSD Kit ELISA, 20-ALPHA-HSD Kit ELISA, C9 Kit ELISA, DD1 Kit ELISA, DD1/DD2 Kit ELISA, DDH Kit ELISA, DDH1 Kit ELISA, H-37 Kit ELISA, HAKRC Kit ELISA, HBAB Kit ELISA, MBAB Kit ELISA, aldo-keto reductase family 1 member C1 Kit ELISA, aldo-keto reductase family 1, member C1 (dihydrodiol dehydrogenase 1; 20-alpha (3-alpha)-hydroxysteroid dehydrogenase) Kit ELISA, AKR1C1 Kit ELISA
ID gène
1645
UniProt
Q04828
Pathways
Steroid Hormone Biosynthesis , C21-Steroid Hormone Metabolic Process
Vus récemment
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