Annexin V Kit ELISA

N° du produit ABIN4881843

Détail du produit Annexin V Kit ELISA

Antigène: Annexin V (ANXA5) (Annexin A5 (ANXA5))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human Annexin A5/Annexin V ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human Cathepsin C.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: Annexin V (ANXA5) (Annexin A5 (ANXA5))

Autre désignation: Annexin A5 (ANXA5 Produits)

Synonymes: anx Kit ELISA, anx5 Kit ELISA, ANX V Kit ELISA, anxa5 Kit ELISA, cb989 Kit ELISA, wu:fa98f06 Kit ELISA, wu:fj10f10 Kit ELISA, MGC89158 Kit ELISA, ANX5 Kit ELISA, ENX2 Kit ELISA, PP4 Kit ELISA, RPRGL3 Kit ELISA, Anx5 Kit ELISA, R74653 Kit ELISA, LC5 Kit ELISA, enx2 Kit ELISA, annexin A5 Kit ELISA, annexin A5b Kit ELISA, Annexin A5 Kit ELISA, annexin A5 L homeolog Kit ELISA, ANXA5 Kit ELISA, anxa5b Kit ELISA, anxa5 Kit ELISA, Anxa5 Kit ELISA, anxa5.L Kit ELISA

ID gène: 308

UniProt: P08758

Pathways: Apoptose