APEX1 Kit ELISA

N° du produit ABIN4881849

Détail du produit APEX1 Kit ELISA

Antigène: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human APE1 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human CD177.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Autre désignation: APE1 (APEX1 Produits)

Synonymes: apex1 Kit ELISA, MGC76280 Kit ELISA, MGC79645 Kit ELISA, MGC78928 Kit ELISA, APE Kit ELISA, APE1 Kit ELISA, APEN Kit ELISA, APEX Kit ELISA, APX Kit ELISA, HAP1 Kit ELISA, REF1 Kit ELISA, Apex Kit ELISA, REF-1 Kit ELISA, BAP1 Kit ELISA, apex1a Kit ELISA, hm:zehn1532 Kit ELISA, wu:fc13d11 Kit ELISA, zgc:66204 Kit ELISA, Ref-1 Kit ELISA, apurinic/apyrimidinic endodeoxyribonuclease 1 Kit ELISA, APEX nuclease (multifunctional DNA repair enzyme) 1 Kit ELISA, APEX nuclease (multifunctional DNA repair enzyme) 1 L homeolog Kit ELISA, DNA-(apurinic or apyrimidinic site) lyase Kit ELISA, apurinic/apyrimidinic endonuclease 1 Kit ELISA, APEX1 Kit ELISA, apex1 Kit ELISA, apex1.L Kit ELISA, CNG03640 Kit ELISA, CNH01280 Kit ELISA, nth Kit ELISA, Spirs_4119 Kit ELISA, Palpr_0103 Kit ELISA, Apex1 Kit ELISA

ID gène: 328

UniProt: P27695

Pathways: Réparation de l'ADN, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus