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B3GNT2 Kit ELISA
B3GNT2
Reactivité: Humain
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4881916
Détail du produit B3GNT2 Kit ELISA
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Antigène
Tous les produits B3GNT2
B3GNT2
(UDP-GlcNAc:BetaGal beta-1,3-N-Acetylglucosaminyltransferase 2 (B3GNT2))
Reactivité
Humain
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Fonction
Custom Human B3GNT2 ELISA Kit.
Type d'échantillon
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Alternatives
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Information d'application
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Volume d'échantillon
100 μL
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Tous les produits B3GNT2
B3GNT2
(UDP-GlcNAc:BetaGal beta-1,3-N-Acetylglucosaminyltransferase 2 (B3GNT2))
Autre désignation
B3GNT2 (B3GNT2 Produits )
Synonymes
B3GN-T2 Kit ELISA, B3GNT Kit ELISA, B3GNT-2 Kit ELISA, B3GNT1 Kit ELISA, BETA3GNT Kit ELISA, BGNT2 Kit ELISA, BGnT-2 Kit ELISA, AA408337 Kit ELISA, AA409404 Kit ELISA, AW260308 Kit ELISA, B3Galt6 Kit ELISA, B3gnt1 Kit ELISA, BGnT-1 Kit ELISA, BGnT1 Kit ELISA, BGnT2 Kit ELISA, Beta3gnt Kit ELISA, MGC81185 Kit ELISA, B3GNT2 Kit ELISA, MGC179257 Kit ELISA, ssp5 Kit ELISA, wu:fa55h04 Kit ELISA, wu:fc39c01 Kit ELISA, zgc:113947 Kit ELISA, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 Kit ELISA, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 L homeolog Kit ELISA, beta-1,4-glucuronyltransferase 1 Kit ELISA, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2b Kit ELISA, B3GNT2 Kit ELISA, B3gnt2 Kit ELISA, b3gnt2.L Kit ELISA, B4GAT1 Kit ELISA, b3gnt2b Kit ELISA, b3gnt2 Kit ELISA
ID gène
10678
UniProt
Q9NY97
Pathways
Glycosaminoglycan Metabolic Process
Vus récemment
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