BMPR1A Kit ELISA

N° du produit ABIN4881978

Détail du produit BMPR1A Kit ELISA

Antigène: BMPR1A (Bone Morphogenetic Protein Receptor 1A (BMPR1A))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human BMPR-1A (ALK-3) ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: The antibody pair provided in this kit recognizes Human BMPR-1A (ALK-3)

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: BMPR1A (Bone Morphogenetic Protein Receptor 1A (BMPR1A))

Autre désignation: BMPR-IA (BMPR1A Produits)

Synonymes: 10q23del Kit ELISA, ACVRLK3 Kit ELISA, ALK3 Kit ELISA, CD292 Kit ELISA, SKR5 Kit ELISA, 1110037I22Rik Kit ELISA, AU045487 Kit ELISA, BMPR-IA Kit ELISA, Bmpr Kit ELISA, BMPR-1 Kit ELISA, BRK-1 Kit ELISA, BMPR1A Kit ELISA, acvrlk3 Kit ELISA, alk-3 Kit ELISA, alk3 Kit ELISA, cd292 Kit ELISA, skr5 Kit ELISA, BR1a Kit ELISA, alk3tr Kit ELISA, bmpr1a Kit ELISA, zgc:136731 Kit ELISA, wu:fe05b06 Kit ELISA, wu:fe06e10 Kit ELISA, zgc:92227 Kit ELISA, bone morphogenetic protein receptor type 1A Kit ELISA, bone morphogenetic protein receptor, type 1A Kit ELISA, bone morphogenetic protein receptor type 1A S homeolog Kit ELISA, bone morphogenetic protein receptor, type IAa Kit ELISA, bone morphogenetic protein receptor, type IAb Kit ELISA, BMPR1A Kit ELISA, Bmpr1a Kit ELISA, bmpr1a Kit ELISA, bmpr1a.S Kit ELISA, bmpr1aa Kit ELISA, bmpr1ab Kit ELISA

ID gène: 657

UniProt: P36894

Pathways: Stem Cell Maintenance