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BORA Kit ELISA
BORA
Reactivité: Humain
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4881987
Détail du produit BORA Kit ELISA
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Antigène
Voir toutes BORA Kits ELISA
BORA
(Protein Aurora Borealis (BORA))
Reactivité
Toutes les réactivités sur BORA Kits ELISA
Humain
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Fonction
Custom Human Bora ELISA Kit.
Type d'échantillon
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificité
This ELISA antibody pair recognizes Human Cystatin SA.
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Alternatives
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Information d'application
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Volume d'échantillon
100 μL
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes BORA Kits ELISA
BORA
(Protein Aurora Borealis (BORA))
Autre désignation
Bora (BORA Produits )
Synonymes
CG6897 Kit ELISA, Dmel\\CG6897 Kit ELISA, zgc:101616 Kit ELISA, C13orf34 Kit ELISA, 6720463M24Rik Kit ELISA, AI317232 Kit ELISA, RGD1309522 Kit ELISA, c13orf34 Kit ELISA, C12H13orf34 Kit ELISA, aurora borealis Kit ELISA, bora, aurora kinase A activator Kit ELISA, protein aurora borealis Kit ELISA, bora, aurora kinase A activator S homeolog Kit ELISA, bora Kit ELISA, Tsp_06066 Kit ELISA, Bora Kit ELISA, BORA Kit ELISA, bora.S Kit ELISA
ID gène
79866
UniProt
Q6PGQ7
Vus récemment
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