CPB1 Kit ELISA

N° du produit ABIN4882085

Détail du produit CPB1 Kit ELISA

Antigène: CPB1 (Carboxypeptidase B1 (Tissue) (CPB1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human Carboxypeptidase B1 (CPB1) ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: CPB1 (Carboxypeptidase B1 (Tissue) (CPB1))

Autre désignation: Carboxypeptidase B1 (CPB1 Produits)

Synonymes: wu:fb60d07 Kit ELISA, wu:fb69b08 Kit ELISA, CPB1 Kit ELISA, pCPB Kit ELISA, CPB Kit ELISA, PASP Kit ELISA, PCPB Kit ELISA, Cpb Kit ELISA, 0910001A18Rik Kit ELISA, 1810063F02Rik Kit ELISA, 2210008M23Rik Kit ELISA, AI504870 Kit ELISA, carboxypeptidase B1 (tissue) Kit ELISA, carboxypeptidase B1 Kit ELISA, carboxypeptidase B1 S homeolog Kit ELISA, carboxypeptidase B Kit ELISA, cpb1 Kit ELISA, CPB1 Kit ELISA, cpb1.S Kit ELISA, CpipJ_CPIJ010799 Kit ELISA, CpipJ_CPIJ010801 Kit ELISA, VDBG_02663 Kit ELISA, LOC100562609 Kit ELISA, Cpb1 Kit ELISA

ID gène: 1360

UniProt: P15086