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CDON Kit ELISA
CDON
Reactivité: Humain
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4882297
Détail du produit CDON Kit ELISA
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Antigène
Voir toutes CDON Kits ELISA
CDON
(Cell Adhesion Molecule-Related/down-Regulated By Oncogenes (CDON))
Reactivité
Toutes les réactivités sur CDON Kits ELISA
Humain
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Fonction
Custom Human CDO ELISA Kit.
Type d'échantillon
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificité
This ELISA antibody pair recognizes Human CDO. Other species not yet determined.
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Information d'application
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Volume d'échantillon
100 μL
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes CDON Kits ELISA
CDON
(Cell Adhesion Molecule-Related/down-Regulated By Oncogenes (CDON))
Autre désignation
CDO (CDON Produits )
Synonymes
CDO Kit ELISA, CDON1 Kit ELISA, HPE11 Kit ELISA, ORCAM Kit ELISA, Cdo Kit ELISA, Cd0 Kit ELISA, Orcam Kit ELISA, cb507 Kit ELISA, wu:fa10c03 Kit ELISA, wu:fb94b11 Kit ELISA, zgc:158240 Kit ELISA, cdon-A Kit ELISA, cell adhesion associated, oncogene regulated Kit ELISA, cell adhesion molecule-related/down-regulated by oncogenes Kit ELISA, cell adhesion associated, oncogene regulated L homeolog Kit ELISA, CDON Kit ELISA, Cdon Kit ELISA, cdon Kit ELISA, cdon.L Kit ELISA
ID gène
1036
UniProt
Q16878
Pathways
Regulation of Muscle Cell Differentiation , Skeletal Muscle Fiber Development , Embryonic Body Morphogenesis
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