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CHST1 Kit ELISA
CHST1
Reactivité: Humain
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4882342
Détail du produit CHST1 Kit ELISA
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Antigène
Voir toutes CHST1 Kits ELISA
CHST1
(Carbohydrate (Keratan Sulfate Gal-6) Sulfotransferase 1 (CHST1))
Reactivité
Toutes les réactivités sur CHST1 Kits ELISA
Humain
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Fonction
Custom Human CHST1 (GST-1) ELISA Kit.
Type d'échantillon
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificité
This ELISA antibody pair recognizes Human CHST1. Other species not yet determined.
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Information d'application
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Volume d'échantillon
100 μL
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes CHST1 Kits ELISA
CHST1
(Carbohydrate (Keratan Sulfate Gal-6) Sulfotransferase 1 (CHST1))
Autre désignation
CHST1 (CHST1 Produits )
Synonymes
C6ST Kit ELISA, GST-1 Kit ELISA, KS6ST Kit ELISA, KSGal6ST Kit ELISA, KSST Kit ELISA, sulfo1 Kit ELISA, 2610008E20Rik Kit ELISA, AW125896 Kit ELISA, Gst1 Kit ELISA, KSGAL6ST Kit ELISA, carbohydrate sulfotransferase 1 Kit ELISA, carbohydrate (keratan sulfate Gal-6) sulfotransferase 1 Kit ELISA, CHST1 Kit ELISA, Chst1 Kit ELISA
ID gène
8534
UniProt
O43916
Pathways
Glycosaminoglycan Metabolic Process
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