c-MYC Kit ELISA

N° du produit ABIN4882399

Détail du produit c-MYC Kit ELISA

Antigène: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.62-150 ng/mL

Seuil minimal de détection: 0.62 ng/mL

Application: ELISA

Fonction: Human c-Myc (MYC) ELISA Kit for serum, plasma, and cell culture supernatant samples.

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: This ELISA antibody pair detects human c-Myc. Other species not determined.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples2 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Autre désignation: C-Myc///MYC (MYC Produits)

Synonymes: MRTL Kit ELISA, MYCC Kit ELISA, bHLHe39 Kit ELISA, c-Myc Kit ELISA, CMYC Kit ELISA, c-myc Kit ELISA, C-MYC Kit ELISA, LOC100136746 Kit ELISA, ARC Kit ELISA, B430311C09Rik Kit ELISA, MYC Kit ELISA, NOP Kit ELISA, Nop30 Kit ELISA, RNCMYC Kit ELISA, mMyc Kit ELISA, AU016757 Kit ELISA, Myc2 Kit ELISA, Niard Kit ELISA, Nird Kit ELISA, cmyc Kit ELISA, zc-myc Kit ELISA, c-myc II Kit ELISA, myc-B Kit ELISA, myc2 Kit ELISA, myc1 Kit ELISA, CMyc Kit ELISA, zgc:55680 Kit ELISA, CG10798 Kit ELISA, D-Myc Kit ELISA, DM Kit ELISA, DMYc Kit ELISA, Dm Kit ELISA, Dmel\CG10798 Kit ELISA, Dmyc Kit ELISA, EG:BACN5I9.1 Kit ELISA, anon-WO03040301.171 Kit ELISA, bHLHe57 Kit ELISA, c-MYC Kit ELISA, d-myc Kit ELISA, dMYC Kit ELISA, dMyc Kit ELISA, dMyc1 Kit ELISA, da Kit ELISA, dm Kit ELISA, dm/dMyc Kit ELISA, dm/myc Kit ELISA, dmyc Kit ELISA, dmyc1 Kit ELISA, l(1)G0139 Kit ELISA, l(1)G0354 Kit ELISA, l(1)G0359 Kit ELISA, myc Kit ELISA, MYC proto-oncogene, bHLH transcription factor Kit ELISA, v-myc myelocytomatosis viral oncogene homolog Kit ELISA, nucleolar protein 3 (apoptosis repressor with CARD domain) Kit ELISA, myelocytomatosis oncogene Kit ELISA, MYC proto-oncogene, bHLH transcription factor a Kit ELISA, MYC proto-oncogene, bHLH transcription factor L homeolog Kit ELISA, MYC proto-oncogene, bHLH transcription factor S homeolog Kit ELISA, v-myc myelocytomatosis viral oncogene homolog (avian) Kit ELISA, MYC proto-oncogene, bHLH transcription factor b Kit ELISA, CG10798 gene product from transcript CG10798-RB Kit ELISA, MYC Kit ELISA, LOC100136746 Kit ELISA, Nol3 Kit ELISA, Myc Kit ELISA, myca Kit ELISA, myc.L Kit ELISA, myc.S Kit ELISA, mycb Kit ELISA

ID gène: 4609

UniProt: P01106

Pathways: Signalisation p53, Cycle Cellulaire, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, L'effet Warburg