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GGCT Kit ELISA
GGCT
Reactivité: Humain
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4882472
Détail du produit GGCT Kit ELISA
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Antigène
Voir toutes GGCT Kits ELISA
GGCT
(gamma-Glutamylcyclotransferase (GGCT))
Reactivité
Toutes les réactivités sur GGCT Kits ELISA
Humain
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Fonction
Custom Human CRF21 ELISA Kit.
Type d'échantillon
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificité
This ELISA antibody pair recognizes Human Myosin light chain 3.
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Information d'application
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Volume d'échantillon
100 μL
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes GGCT Kits ELISA
GGCT
(gamma-Glutamylcyclotransferase (GGCT))
Autre désignation
CRF21 (GGCT Produits )
Synonymes
fj35c05 Kit ELISA, ggct Kit ELISA, wu:fj35b07 Kit ELISA, wu:fj35c05 Kit ELISA, zgc:56140 Kit ELISA, A030007L17Rik Kit ELISA, AA673177 Kit ELISA, Gctg Kit ELISA, Ggc Kit ELISA, Ggctl1 Kit ELISA, RGD1304876 Kit ELISA, RGD1562590 Kit ELISA, C7orf24 Kit ELISA, CRF21 Kit ELISA, GCTG Kit ELISA, GGC Kit ELISA, gamma-glutamylcyclotransferase b Kit ELISA, gamma-glutamylcyclotransferase Kit ELISA, gamma-glutamyl cyclotransferase Kit ELISA, ggctb Kit ELISA, GGCT Kit ELISA, ggct Kit ELISA, Ggct Kit ELISA
ID gène
79017
UniProt
O75223
Vus récemment
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