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GGCT Kit ELISA

GGCT Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4882472
  • Antigène Voir toutes GGCT Kits ELISA
    GGCT (gamma-Glutamylcyclotransferase (GGCT))
    Reactivité
    • 2
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Custom Human CRF21 ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair recognizes Human Myosin light chain 3.
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes GGCT Kits ELISA
    GGCT (gamma-Glutamylcyclotransferase (GGCT))
    Autre désignation
    CRF21 (GGCT Produits)
    Synonymes
    fj35c05 Kit ELISA, ggct Kit ELISA, wu:fj35b07 Kit ELISA, wu:fj35c05 Kit ELISA, zgc:56140 Kit ELISA, A030007L17Rik Kit ELISA, AA673177 Kit ELISA, Gctg Kit ELISA, Ggc Kit ELISA, Ggctl1 Kit ELISA, RGD1304876 Kit ELISA, RGD1562590 Kit ELISA, C7orf24 Kit ELISA, CRF21 Kit ELISA, GCTG Kit ELISA, GGC Kit ELISA, gamma-glutamylcyclotransferase b Kit ELISA, gamma-glutamylcyclotransferase Kit ELISA, gamma-glutamyl cyclotransferase Kit ELISA, ggctb Kit ELISA, GGCT Kit ELISA, ggct Kit ELISA, Ggct Kit ELISA
    ID gène
    79017
    UniProt
    O75223
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