CFC1 Kit ELISA

N° du produit ABIN4882495

Détail du produit CFC1 Kit ELISA

Antigène: CFC1 (Cripto, FRL-1, Cryptic Family 1 (CFC1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human Cryptic ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human Cryptic. Other species not yet determined.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: CFC1 (Cripto, FRL-1, Cryptic Family 1 (CFC1))

Autre désignation: Cryptic (CFC1 Produits)

Synonymes: CFC1B Kit ELISA, RGD1562188 Kit ELISA, Cripto-1 Kit ELISA, FRL-1 Kit ELISA, cfc1 Kit ELISA, cr1 Kit ELISA, cripto Kit ELISA, frl1 Kit ELISA, tdgf1 Kit ELISA, xcr1 Kit ELISA, CRYPTIC Kit ELISA, DTGA2 Kit ELISA, HTX2 Kit ELISA, AV265756 Kit ELISA, b2b970Clo Kit ELISA, cryptic Kit ELISA, CFC1 Kit ELISA, cryptic family protein 1B Kit ELISA, cripto, FRL-1, cryptic family 1 Kit ELISA, teratocarcinoma-derived growth factor 1, member 3 Kit ELISA, LOC459608 Kit ELISA, Cfc1 Kit ELISA, CFC1 Kit ELISA, tdgf1.3 Kit ELISA

ID gène: 55997

UniProt: P0CG37