Cyclin E1 Kit ELISA

N° du produit ABIN4882515

Détail du produit Cyclin E1 Kit ELISA

Antigène: Cyclin E1 (CCNE1)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human Cyclin E1 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human Nicastrin.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: Cyclin E1 (CCNE1)

Autre désignation: Cyclin E1 (CCNE1 Produits)

Synonymes: CCNE1 Kit ELISA, ccne Kit ELISA, cyclinE Kit ELISA, CCNE Kit ELISA, AW538188 Kit ELISA, CycE1 Kit ELISA, CYCLE Kit ELISA, Ccne Kit ELISA, cyce2 Kit ELISA, cycE3 Kit ELISA, cyce Kit ELISA, fa18e07 Kit ELISA, wu:fa18e07 Kit ELISA, cyclin E1 Kit ELISA, cyclin E1 L homeolog Kit ELISA, cyclin E1 S homeolog Kit ELISA, CCNE1 Kit ELISA, ccne1 Kit ELISA, Ccne1 Kit ELISA, ccne1.L Kit ELISA, ccne1.S Kit ELISA

ID gène: 898

UniProt: P24864

Pathways: Cycle Cellulaire, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases