DC-SIGN/CD209 Kit ELISA

N° du produit ABIN4882572

Détail du produit DC-SIGN/CD209 Kit ELISA

Antigène: DC-SIGN/CD209 (CD209) (CD209)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human DC-SIGN/CD209 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human PlGF-2.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: DC-SIGN/CD209 (CD209) (CD209)

Autre désignation: DC-SIGN (CD209 Produits)

Synonymes: CDSIGN Kit ELISA, CLEC4L Kit ELISA, DC-SIGN Kit ELISA, DC-SIGN1 Kit ELISA, cd209 Kit ELISA, CLEC4M Kit ELISA, si:ch211-224h1.3 Kit ELISA, CD209 Kit ELISA, CIRE Kit ELISA, Dcsign Kit ELISA, SIGN-R1 Kit ELISA, SIGNR5 Kit ELISA, Cd209 Kit ELISA, CD209 molecule Kit ELISA, CD209a antigen Kit ELISA, CD209 antigen-like protein D Kit ELISA, CD209c molecule Kit ELISA, CD209 antigen Kit ELISA, CD209 Kit ELISA, cd209 Kit ELISA, Cd209a Kit ELISA, LOC100529184 Kit ELISA, Cd209c Kit ELISA, LOC100460708 Kit ELISA, LOC105484282 Kit ELISA

ID gène: 30835

UniProt: Q9NNX6