DDC Kit ELISA

N° du produit ABIN4882581

Détail du produit DDC Kit ELISA

Antigène: DDC (Dopa Decarboxylase (Aromatic L-Amino Acid Decarboxylase) (DDC))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human Dopa Decarboxylase (DDC) ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human Dopa Decarboxylase. Other species not yet determined.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: DDC (Dopa Decarboxylase (Aromatic L-Amino Acid Decarboxylase) (DDC))

Autre désignation: Dopa Decarboxylase (DDC Produits)

Synonymes: AADC Kit ELISA, wu:fa56d05 Kit ELISA, wu:fd59h03 Kit ELISA, wu:fk20h01 Kit ELISA, zgc:65801 Kit ELISA, zgc:76929 Kit ELISA, Ddc Kit ELISA, DdcDv Kit ELISA, Dvir\\GJ17995 Kit ELISA, GJ17995 Kit ELISA, dvir_GLEANR_2561 Kit ELISA, Aadc Kit ELISA, 5-HT Kit ELISA, CG10697 Kit ELISA, DDC Kit ELISA, DdcDm Kit ELISA, Dmel\\CG10697 Kit ELISA, ddc Kit ELISA, fDDC Kit ELISA, l(2)37Bl Kit ELISA, l(2)37Ch Kit ELISA, l(2)k02104 Kit ELISA, dopa decarboxylase Kit ELISA, Dopa-decarboxylase Kit ELISA, dopa decarboxylase (aromatic L-amino acid decarboxylase) Kit ELISA, Dopa decarboxylase Kit ELISA, dopa decarboxylase L homeolog Kit ELISA, DDC Kit ELISA, ddc Kit ELISA, Ddc Kit ELISA, Dvir\Ddc Kit ELISA, ddc.L Kit ELISA

ID gène: 1644

UniProt: P20711

Pathways: Dopaminergic Neurogenesis