Desmin Kit ELISA

N° du produit ABIN4882602

Détail du produit Desmin Kit ELISA

Antigène: Desmin (DES)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human Desmin ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human Desmin.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: Desmin (DES)

Autre désignation: Desmin (DES Produits)

Synonymes: DES Kit ELISA, des-b Kit ELISA, MGC80853 Kit ELISA, des Kit ELISA, csm1 Kit ELISA, csm2 Kit ELISA, desm Kit ELISA, cmd1i Kit ELISA, MGC75911 Kit ELISA, LOC100220724 Kit ELISA, desmin Kit ELISA, cb290 Kit ELISA, fb59a12 Kit ELISA, wu:fb59a12 Kit ELISA, zgc:109859 Kit ELISA, CSM1 Kit ELISA, CSM2 Kit ELISA, LGMD2R Kit ELISA, wu:fc11d08 Kit ELISA, zgc:154009 Kit ELISA, des-a Kit ELISA, MGC52614 Kit ELISA, desmin Kit ELISA, desmin, gene 1 L homeolog Kit ELISA, desmin, gene 1 Kit ELISA, desmin a Kit ELISA, desmin b Kit ELISA, desmin, gene 1 S homeolog Kit ELISA, desmin, gene 2 S homeolog Kit ELISA, DES Kit ELISA, des.1.L Kit ELISA, des.1 Kit ELISA, des Kit ELISA, desma Kit ELISA, Des Kit ELISA, desmb Kit ELISA, des.1.S Kit ELISA, des.2.S Kit ELISA

ID gène: 1674

UniProt: P17661